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Literature summary for 4.1.3.4 extracted from
- The enzymatic cleavage of beta-hydroxy-beta-methylglutaryl coenzyme A to acetoacetate and acetyl coenzyme A (1955), J. Biol. Chem., 216, 727-736.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiols | required, e.g. cysteine | Sus scrofa |  |
| thiols | required, e.g. cysteine | Columba livia | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Columba livia | |
| EDTA | - |
Sus scrofa | |
| Thiomalic acid | - |
Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, can be substituted by Mn2+ | Sus scrofa | |
| Mg2+ | required, can be substituted by Mn2+ | Columba livia | |
| Mn2+ | required, can be substituted by Mg2+ | Sus scrofa | |
| Mn2+ | required, can be substituted by Mg2+ | Columba livia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Columba livia | - |
pigeon | - |
| Sus scrofa | - |
pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
- |
Columba livia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Sus scrofa | - |
| kidney | - |
Sus scrofa | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Columba livia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Columba livia |
| additional information | - |
tissue distribution of activities | Sus scrofa |
| 7 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl buffer, pH7.4, 1 mM cysteine, several months | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | stereochemistry | Sus scrofa | Acetyl-CoA + acetoacetate | - |
? | |
| (S)-3-Hydroxy-3-methylglutaryl-CoA | stereochemistry | Columba livia | Acetyl-CoA + acetoacetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Sus scrofa |
| 37 | - |
enzyme assay at | Columba livia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
enzyme assay at | Columba livia |
| 10 | - |
- |
Sus scrofa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 10.5 | - |
Sus scrofa |
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