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Literature summary for 4.1.3.39 extracted from

  • Powlowski, J.; Sahlman, L.; Shingler, V.
    Purification and properties of the physically associated meta-cleavage pathway enzymes 4-hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating) from Pseudomonas sp. strain CF600 (1993), J. Bacteriol., 175, 377-385.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.39

Activating Compound

Activating Compound Comment Organism Structure
NAD+ stimulates slightly Pseudomonas sp.
NADH stimulates Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene dmpG encodes the enzyme in the dmp operon together with aldehyde dehydrogenase, EC 1.2.1.10, with which it forms an enzyme complex Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ strong inhibition Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates 6-8fold at 1 mM Pseudomonas sp.
additional information no effect by Mg2+ and Ca2+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000 + 2 * 40000, enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE Pseudomonas sp.
40000
-
 2 * 35000 + 2 * 40000, enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE Pseudomonas sp.
148000
-
 enzyme in complex with aldehyde dehydrogenase, EC 1.2.1.10, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxy-2-oxovalerate Pseudomonas sp.
-
 pyruvate + acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 gene dmpG
-
Pseudomonas sp. CF 600
-
 gene dmpG
-

Purification (Commentary)

Purification (Comment) Organism
co-purification with aldehyde dehydrogenase, EC 1.2.1.10, to homogeneity in a 5-step chromatographic procedure Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2
-
 purified enzyme Pseudomonas sp.

Storage Stability

Storage Stability Organism
-80°C, purified native enzyme, stable for at least 6 months Pseudomonas sp.
4-6°C, purified native enzyme, stable for at least 6 days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-2-oxovalerate
-
 Pseudomonas sp. pyruvate + acetaldehyde
-
 ?
4-hydroxy-2-oxovalerate the enzyme utilizes the L-(S)-isomer or the racemate Pseudomonas sp. pyruvate + acetaldehyde
-
 ?
additional information the enzyme acts in an enzyme complex with the aldehyde dehydrogenase, EC 1.2.1.10, which acts on acetaldehyde to form acetyl-CoA Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme is encoded in the dmp operon with aldehyde dehydrogenase, EC 1.2.1.10, with which it forms an enzyme complex Pseudomonas sp.
tetramer 2 * 35000 + 2 * 40000, enzyme complex of aldehyde dehydrogenase and 4-hydroxy-2-ketovalerate aldolase, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
4-hydroxy-2-ketovalerate aldolase
-
 Pseudomonas sp.
HOA
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
 Pseudomonas sp.