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Literature summary for 4.1.3.36 extracted from
- Ligand-dependent active-site closure revealed in the crystal structure of Mycobacterium tuberculosis MenB complexed with product analogues (2014), Acta Crystallogr. Sect. D, 70, 2959-2969 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with 1-hydroxy-2-naphthoyl-CoA or salicylyl-CoA, hanging drop vapor diffusion method, using 0.3 M NaCl, 0.1 M Tris-HCl pH 8.0, 25% (w/v) PEG 3350 | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-succinylbenzoyl-CoA | Mycobacterium tuberculosis | - |
1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? |  |
| o-succinylbenzoyl-CoA | Mycobacterium tuberculosis H37Rv | - |
1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WNP5 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WNP5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap chelating column chromatography and Sephacryl S-100 gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-succinylbenzoyl-CoA | - |
Mycobacterium tuberculosis | 1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? | |
| o-succinylbenzoyl-CoA | - |
Mycobacterium tuberculosis H37Rv | 1,4-dihydroxy-2-naphthoyl-CoA + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,4-dihydroxy-2-naphthoyl coenzyme A synthase | - |
Mycobacterium tuberculosis |
| DHNA-CoA synthase | - |
Mycobacterium tuberculosis |
| MenB | - |
Mycobacterium tuberculosis |
| Rv0548c | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is cofactor-free | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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