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Literature summary for 4.1.3.36 extracted from

  • Li, H.J.; Li, X.; Liu, N.; Zhang, H.; Truglio, J.J.; Mishra, S.; Kisker, C.F.; Garcia-Diaz, M.; Tonge, P.J.
    Mechanism of the intramolecular Claisen condensation reaction catalyzed by MenB, a crotonase superfamily member (2011), Biochemistry, 50, 9532-9544.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.36

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Mycobacterium tuberculosis
His-tagged version expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion technique, PEG 6000, in complex with o-succinylbenzoyl-aminoCoA Mycobacterium tuberculosis
sitting drop vapor diffusion technique, PEG 3350, in complex with o-succinylbenzoyl-amino-CoA Escherichia coli

Protein Variants

Protein Variants Comment Organism
D185E reduced kcat Mycobacterium tuberculosis
D185G no activity Mycobacterium tuberculosis
D185N reduced kcat Mycobacterium tuberculosis
D192N no activity Mycobacterium tuberculosis
G156D no activity Escherichia coli
S190A reduced kcat Mycobacterium tuberculosis
Y287F no activity Mycobacterium tuberculosis
Y97F no activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.0048
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.0224
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.0259
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
0.0402
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.106
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 6 * 30000, SDS-PAGE, crystallization Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis P9WNP5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis
His-tag affinity chromatography, tag removed Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(3-carboxypropyl)-benzoyl-CoA
-
 Mycobacterium tuberculosis ?
-
 ?
2-succinylbenzoyl-CoA
-
 Mycobacterium tuberculosis 1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
 ?
o-succinylbenzoyl-CoA
-
 Escherichia coli 1,4-dihydroxy-2-naphthoyl-CoA + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer crystallization Mycobacterium tuberculosis
hexamer 6 * 30000, SDS-PAGE, crystallization Escherichia coli

Synonyms

Synonyms Comment Organism
1,4-dihydroxy-2-naphthoyl-CoA synthase
-
 Escherichia coli
1,4-dihydroxy-2-naphthoyl-CoA synthase
-
 Mycobacterium tuberculosis
MenB
-
 Escherichia coli
MenB
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00022
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.00025
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.0017
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.0023
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.062
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
0.462
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0024
-
 2-(3-carboxypropyl)-benzoyl-CoA wild type protein protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.04
-
 2-succinylbenzoyl-CoA S190A mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.07
-
 2-succinylbenzoyl-CoA D185N mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
0.48
-
 2-succinylbenzoyl-CoA D185E mutant protein, pH 7.0, 25°C Mycobacterium tuberculosis
2.4
-
 o-succinylbenzoyl-CoA pH 7.0, 25°C Escherichia coli
21
-
 2-succinylbenzoyl-CoA wild type protein, pH 7.0, 25°C Mycobacterium tuberculosis