Literature summary for 4.1.3.36 extracted from

Kanaujia, S.P.; Ranjani, C.V.; Jeyakanthan, J.; Baba, S.; Kuroishi, C.; Ebihara, A.; Shinkai, A.; Kuramitsu, S.; Shiro, Y.; Sekar, K.; Yokoyama, S.
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus (2007), Acta Crystallogr. Sect. F, 63, 103-105.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21-CodonPlus(DE3)-RIL	Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method with 30% (v/v) pentaerythritol ethocylate, 0.05 M ammonium sulfate, and 0.05 M bis-Tris pH 6.5	Geobacillus kaustophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	3 * 30000, gel filtration	Geobacillus kaustophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap Chelating HP5 Superdex 200 gel filtration and HiLoad 16/60 Superdex 200 gel filtration	Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
o-succinylbenzoyl-CoA	-	Geobacillus kaustophilus	1,4-dihydroxy-2-naphthoyl-CoA + H2O	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 30000, gel filtration	Geobacillus kaustophilus

Synonyms

Synonyms	Comment	Organism
1,4-dihydroxy-2-naphthoate synthetase	-	Geobacillus kaustophilus
DHNA synthetase	-	Geobacillus kaustophilus
MenB	-	Geobacillus kaustophilus

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Release 2023.1 (January 2023)