Any feedback?
Literature summary for 4.1.3.34 extracted from
- A novel enzyme, citryl-CoA lyase, catalysing the second step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK-6 (2004), Mol. Microbiol., 52, 763-770.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned, sequenced and expressed in Escherichia coli BL21(DE3) | Hydrogenobacter thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Hydrogenobacter thermophilus |  |
| citrate | - |
Hydrogenobacter thermophilus | |
| CoA | - |
Hydrogenobacter thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
acetyl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
| 0.082 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
| 0.13 | - |
oxaloacetate | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
| 87000 | - |
gel filtration | Hydrogenobacter thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-citryl-CoA | Hydrogenobacter thermophilus | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r | |
| (3S)-citryl-CoA | Hydrogenobacter thermophilus TK-6 / IAM 12695 | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | acetyl-CoA + oxaloacetate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenobacter thermophilus | - |
- |
- |
| Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant enzyme | Hydrogenobacter thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
citrate synthase activity as a side reaction | Hydrogenobacter thermophilus |
| 33 | - |
citrate, ATP, CoA and citryl-CoA forming enzyme CCS in the enzyme assay | Hydrogenobacter thermophilus |
| 280 | - |
purified citryl-CoA as substrate | Hydrogenobacter thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-citryl-CoA | - |
Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
| (3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus | acetyl-CoA + oxaloacetate | - |
r | |
| (3S)-citryl-CoA | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
| (3S)-citryl-CoA | reductive tricarboxylic acid cycle, second step of citrate cleavage reaction | Hydrogenobacter thermophilus TK-6 / IAM 12695 | acetyl-CoA + oxaloacetate | - |
r | |
| additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus | ? | - |
? | |
| additional information | shows low citrate synthase activity | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 30000, homotrimer, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCL | - |
Hydrogenobacter thermophilus |
| citryl-CoA lyase | - |
Hydrogenobacter thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 90 | thermostable with no detectable loss of activity at 80°C for 10 min, retains 83% after 90°C for 10 min | Hydrogenobacter thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 570 | - |
citryl-CoA | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Hydrogenobacter thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | 10% of maximum activity at pH 7.0, 50% of maximum activity at pH 7.5, 90% of maximum activity at pH 9.0, 30% of maximum activity at pH 9.5 | Hydrogenobacter thermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
