Literature summary for 4.1.3.27 extracted from

Bashiri, G.; Johnston, J.M.; Evans, G.L.; Bulloch, E.M.; Goldstone, D.C.; Jirgis, E.N.; Kleinboelting, S.; Castell, A.; Ramsay, R.J.; Manos-Turvey, A.; Payne, R.J.; Lott, J.S.; Baker, E.N.
Structure and inhibition of subunit I of the anthranilate synthase complex of Mycobacterium tuberculosis and expression of the active complex (2015), Acta Crystallogr. Sect. D, 71, 2297-2308 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 150 mM trisodium citrate pH 5.5, 0.75-1.5 M ammonium sulfate, 0.25-0.5 M lithium sulfate	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
3-(1-carboxyethoxy)-4,5-dihydroxybenzoate	-	Mycobacterium tuberculosis
3-[(1-carboxylatoethenyl)oxy]-2-hydroxybenzoate	-	Mycobacterium tuberculosis
3-[(1-carboxylatoethenyl)oxy]benzoate	-	Mycobacterium tuberculosis
3-[(2-carboxyprop-2-en-1-yl)oxy]-4,5-dihydroxybenzoate	-	Mycobacterium tuberculosis
3-[[(1E)-1-carboxylatobut-1-en-1-yl]oxy]-2-hydroxybenzoate	-	Mycobacterium tuberculosis
3-[[(1E)-1-carboxylatoprop-1-en-1-yl]oxy]-2-hydroxybenzoate	-	Mycobacterium tuberculosis
3-[[(E)-1-carboxylato-2-phenylethenyl]oxy]-2-hydroxybenzoate	-	Mycobacterium tuberculosis
tryptophan	allosteric inhibition	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	chorismate	at pH 8.0 and 25°C	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chorismate + L-glutamine	Mycobacterium tuberculosis	-	anthranilate + pyruvate + L-glutamate	-	?
chorismate + L-glutamine	Mycobacterium tuberculosis CDC 1551	-	anthranilate + pyruvate + L-glutamate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WFX2	-	-
Mycobacterium tuberculosis CDC 1551	P9WFX2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chorismate + L-glutamine	-	Mycobacterium tuberculosis	anthranilate + pyruvate + L-glutamate	-	?
chorismate + L-glutamine	-	Mycobacterium tuberculosis CDC 1551	anthranilate + pyruvate + L-glutamate	-	?

Subunits

Subunits	Comment	Organism
?	x * 54000, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
AS-I	-	Mycobacterium tuberculosis
TrpE	-	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.26	-	chorismate	at pH 8.0 and 25°C	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0063	-	at pH 8.0 and 25°C	Mycobacterium tuberculosis	tryptophan

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Release 2023.1 (January 2023)