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Literature summary for 4.1.3.27 extracted from

  • Tutino, M.L.; Tosco, A.; Marino, G.; Sannia, G.
    Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli (1997), Biochem. Biophys. Res. Commun., 230, 306-310.
    View publication on PubMed
Search for more literature for 4.1.3.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus

General Stability

General Stability Organism
1 mM dithiothreitol and 50 mM L-glutamine have a stabilizing effect each Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
L-tryptophan
-
 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 chorismate in 50 mM potassium phosphate buffer (pH 7.5), 20 mM L-glutamine, and 10 mM MgCl2, at 60°C Saccharolobus solfataricus
0.0093
-
 NH4+ in 50 mM Tris buffer (pH 8.0), 50 mM NH4Cl, and 10 mM MgCl2, at 60°C Saccharolobus solfataricus
0.0193
-
 L-Gln in 50 mM potassium phosphate buffer (pH 7.5), 20 mM L-glutamine, and 10 mM MgCl2, at 60°C Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21913
-
 2 * 47743 + 2 * 21913, deduced from amino acid sequence Saccharolobus solfataricus
47743
-
 2 * 47743 + 2 * 21913, deduced from amino acid sequence Saccharolobus solfataricus
122000
-
 gel filtration Saccharolobus solfataricus
139300
-
 deduced from amino acid sequence Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus MT4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
 Saccharolobus solfataricus anthranilate + pyruvate + L-glutamate
-
 ?
chorismate + L-Gln
-
 Saccharolobus solfataricus MT4 anthranilate + pyruvate + L-glutamate
-
 ?
chorismate + NH4+
-
 Saccharolobus solfataricus ?
-
 ?
chorismate + NH4+
-
 Saccharolobus solfataricus MT4 ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 47743 + 2 * 21913, deduced from amino acid sequence Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 85 activity steadily increases with the rising of temperature in the range 25°C to 85°C, the enzyme is fully active above 85°C Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
 L-tryptophan at 60°C Saccharolobus solfataricus