Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
1 mM dithiothreitol and 50 mM L-glutamine have a stabilizing effect each | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tryptophan | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
chorismate | in 50 mM potassium phosphate buffer (pH 7.5), 20 mM L-glutamine, and 10 mM MgCl2, at 60°C | Saccharolobus solfataricus | |
0.0093 | - |
NH4+ | in 50 mM Tris buffer (pH 8.0), 50 mM NH4Cl, and 10 mM MgCl2, at 60°C | Saccharolobus solfataricus | |
0.0193 | - |
L-Gln | in 50 mM potassium phosphate buffer (pH 7.5), 20 mM L-glutamine, and 10 mM MgCl2, at 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21913 | - |
2 * 47743 + 2 * 21913, deduced from amino acid sequence | Saccharolobus solfataricus |
47743 | - |
2 * 47743 + 2 * 21913, deduced from amino acid sequence | Saccharolobus solfataricus |
122000 | - |
gel filtration | Saccharolobus solfataricus |
139300 | - |
deduced from amino acid sequence | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus MT4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-Gln | - |
Saccharolobus solfataricus | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-Gln | - |
Saccharolobus solfataricus MT4 | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + NH4+ | - |
Saccharolobus solfataricus | ? | - |
? | |
chorismate + NH4+ | - |
Saccharolobus solfataricus MT4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 47743 + 2 * 21913, deduced from amino acid sequence | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 85 | activity steadily increases with the rising of temperature in the range 25°C to 85°C, the enzyme is fully active above 85°C | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
L-tryptophan | at 60°C | Saccharolobus solfataricus |