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Literature summary for 4.1.3.27 extracted from
- Characterization of Bacillus caldotenax anthranilate synthase I produced in Escherichia coli and identification of its essential arginine residue by site-directed mutagenesis (1992), J. Biochem., 112, 714-718.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R321H | Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number | [Bacillus] caldotenax |
| R358H | Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number | [Bacillus] caldotenax |
| R429H | similar Km value to the wild-type enzyme and a slightly larger turnover number | [Bacillus] caldotenax |
| R452H | less than 0.05% of the activity of the wild-type enzyme | [Bacillus] caldotenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Trp | - |
[Bacillus] caldotenax |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
chorismate | at pH 9 and 37°C, wild-type enzyme | [Bacillus] caldotenax | |
| 0.042 | - |
chorismate | mutant enzyme R429H | [Bacillus] caldotenax | |
| 0.14 | - |
chorismate | mutant enzyme R358H and R416H | [Bacillus] caldotenax | |
| 0.15 | - |
chorismate | mutant enzyme R321H | [Bacillus] caldotenax | |
| 46 | - |
NH4+ | at pH 9 and 37°C | [Bacillus] caldotenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | [Bacillus] caldotenax | |
| Mg2+ | maximal activity at 10 mM | [Bacillus] caldotenax | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Bacillus] caldotenax | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anthranilate synthase I, from a plasmid bearing Escherichia coli | [Bacillus] caldotenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
[Bacillus] caldotenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-Gln | - |
[Bacillus] caldotenax | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + NH4+ | anthranilate synthase I | [Bacillus] caldotenax | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
[Bacillus] caldotenax |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 383 | - |
chorismate | at pH 9 and 37°C, with NH4+ as cosubstrate | [Bacillus] caldotenax | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
[Bacillus] caldotenax |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: about 20% of maximal activity, pH 9.0: optimum | [Bacillus] caldotenax |
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