Protein Variants | Comment | Organism |
---|---|---|
R321H | Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number | [Bacillus] caldotenax |
R358H | Km-value is increased 3fold to 4fold compared to the value for the wild-type enzyme, little effect on turnover number | [Bacillus] caldotenax |
R429H | similar Km value to the wild-type enzyme and a slightly larger turnover number | [Bacillus] caldotenax |
R452H | less than 0.05% of the activity of the wild-type enzyme | [Bacillus] caldotenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Trp | - |
[Bacillus] caldotenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
chorismate | at pH 9 and 37°C, wild-type enzyme | [Bacillus] caldotenax | |
0.042 | - |
chorismate | mutant enzyme R429H | [Bacillus] caldotenax | |
0.14 | - |
chorismate | mutant enzyme R358H and R416H | [Bacillus] caldotenax | |
0.15 | - |
chorismate | mutant enzyme R321H | [Bacillus] caldotenax | |
46 | - |
NH4+ | at pH 9 and 37°C | [Bacillus] caldotenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | [Bacillus] caldotenax | |
Mg2+ | maximal activity at 10 mM | [Bacillus] caldotenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldotenax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anthranilate synthase I, from a plasmid bearing Escherichia coli | [Bacillus] caldotenax |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Bacillus] caldotenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-Gln | - |
[Bacillus] caldotenax | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + NH4+ | anthranilate synthase I | [Bacillus] caldotenax | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
[Bacillus] caldotenax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
383 | - |
chorismate | at pH 9 and 37°C, with NH4+ as cosubstrate | [Bacillus] caldotenax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
[Bacillus] caldotenax |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 20% of maximal activity, pH 9.0: optimum | [Bacillus] caldotenax |