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Literature summary for 4.1.3.27 extracted from
- Anthranilate synthase from Ailanthus altissima cells suspension cultures (1996), Phytochemistry, 41, 395-402.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Trp | isoenzyme AS-b is inhibited 97% by 0.020 mM, isoenzyme AS-a is inhibited 66% by 0.020 mM | Ailanthus altissimus |  |
| L-Trp | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Gln | isoenzyme As-b | Ailanthus altissimus | |
| 0.073 | - |
chorismate | isoenzyme As-b | Ailanthus altissimus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| protoplast | - |
Ailanthus altissimus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Mg2+ | required | Ailanthus altissimus | |
| Mg2+ | Km: 0.18 mM | Ailanthus altissimus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
1 * 37000 + 1 * 60000, isoenzyme AS-b, SDS-PAGE | Ailanthus altissimus |
| 60000 | - |
1 * 37000 + 1 * 60000, isoenzyme AS-b, SDS-PAGE | Ailanthus altissimus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ailanthus altissimus | - |
two isoenzymes: AS-a and AS-b | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme As-b | Ailanthus altissimus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ailanthus altissimus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + L-Gln | - |
Salmonella enterica subsp. enterica serovar Typhimurium | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + L-Gln | - |
Ailanthus altissimus | anthranilate + pyruvate + L-glutamate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 37000 + 1 * 60000, isoenzyme AS-b, SDS-PAGE | Ailanthus altissimus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Ailanthus altissimus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 8 | isoenzyme AS-b | Ailanthus altissimus |
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