Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anthranilate | - |
Streptomyces sp. | |
L-Trp | - |
Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 28000 + 1 * 72000, gel filtration after separation of subunits | Streptomyces sp. |
72000 | - |
1 * 28000 + 1 * 72000, gel filtration after separation of subunits | Streptomyces sp. |
94000 | 117000 | gel filtration | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptomyces sp. | synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate | ? | - |
? | |
additional information | Streptomyces sp. 3022a | synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. 3022a | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces sp. |
Storage Stability | Organism |
---|---|
-20°C, activity of partially purified enzyme declines during storage | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-Gln | - |
Streptomyces sp. | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + L-Gln | - |
Streptomyces sp. 3022a | anthranilate + pyruvate + L-glutamate | - |
? | |
chorismate + NH4+ | - |
Streptomyces sp. | ? | - |
? | |
chorismate + NH4+ | - |
Streptomyces sp. 3022a | ? | - |
? | |
additional information | the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate | Streptomyces sp. | ? | - |
? | |
additional information | synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate | Streptomyces sp. | ? | - |
? | |
additional information | the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate | Streptomyces sp. 3022a | ? | - |
? | |
additional information | synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate | Streptomyces sp. 3022a | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 28000 + 1 * 72000, gel filtration after separation of subunits | Streptomyces sp. |
More | the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
NH4+-dependent activity increases linearly through pH 9.0 without reaching a maximum | Streptomyces sp. |
7.2 | 7.6 | Gln-dependent activity | Streptomyces sp. |