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Literature summary for 4.1.3.27 extracted from

  • Francis, M.M.; Vining, L.C.; Westlake, D.W.S.
    Characterization and regulation of anthranilate synthetase from a chloramphenicol-producing streptomycete (1978), J. Bacteriol., 134, 10-16.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
anthranilate
-
Streptomyces sp.
L-Trp
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
72000
-
1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
94000 117000 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptomyces sp. synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate ?
-
?
additional information Streptomyces sp. 3022a synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. 3022a
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces sp.

Storage Stability

Storage Stability Organism
-20°C, activity of partially purified enzyme declines during storage Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chorismate + L-Gln
-
Streptomyces sp. anthranilate + pyruvate + L-glutamate
-
?
chorismate + L-Gln
-
Streptomyces sp. 3022a anthranilate + pyruvate + L-glutamate
-
?
chorismate + NH4+
-
Streptomyces sp. ?
-
?
chorismate + NH4+
-
Streptomyces sp. 3022a ?
-
?
additional information the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp. ?
-
?
additional information synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate Streptomyces sp. ?
-
?
additional information the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp. 3022a ?
-
?
additional information synthesis of the enzyme is repressed by His, anthranilate, Trp and p-aminobenzoate Streptomyces sp. 3022a ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 28000 + 1 * 72000, gel filtration after separation of subunits Streptomyces sp.
More the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
NH4+-dependent activity increases linearly through pH 9.0 without reaching a maximum Streptomyces sp.
7.2 7.6 Gln-dependent activity Streptomyces sp.