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Literature summary for 4.1.3.24 extracted from
- Purification and properties of malyl-coenzyme A lyase from Pseudomonas AM1 (1974), Biochem. J., 139, 399-405.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (2R)-4-malyl-CoA | - |
Methylorubrum extorquens |  |
| iodoacetate | - |
Methylorubrum extorquens | |
| NEM | - |
Methylorubrum extorquens | |
| PCMB | - |
Methylorubrum extorquens | |
| Tris | - |
Methylorubrum extorquens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
acetyl-CoA | - |
Methylorubrum extorquens | |
| 0.066 | - |
(3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Methylorubrum extorquens | |
| 1.7 | - |
glyoxylate | - |
Methylorubrum extorquens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required, Mg2+ or Co2+ is most effective | Methylorubrum extorquens | |
| Mg2+ | Km: 1.2 mM | Methylorubrum extorquens | |
| Mg2+ | divalent cation required, Mg2+ or Co2+ is most effective | Methylorubrum extorquens | |
| Mn2+ | divalent cation required, 88% of the activation with Mg2+ and Co2+ | Methylorubrum extorquens | |
| Zn2+ | divalent cation required, 41% of the activation with Mg2+ and Co2+ | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
meniscus depletion method | Methylorubrum extorquens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | - |
- |
- |
| Pseudomonas sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylorubrum extorquens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas sp. |
| additional information | - |
- |
Methylorubrum extorquens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, stable for several months | Methylorubrum extorquens |
| 0-4°C, 2-3 weeks, stable | Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Pseudomonas sp. | acetyl-CoA + glyoxylate | - |
r | |
| (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
Methylorubrum extorquens | acetyl-CoA + glyoxylate | - |
r | |
| acetyl-CoA + glyoxylate | - |
Pseudomonas sp. | (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
r | |
| acetyl-CoA + glyoxylate | - |
Methylorubrum extorquens | (3S)-3-carboxy-3-hydroxypropanoyl-CoA | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
irreversible loss of activity | Methylorubrum extorquens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Methylorubrum extorquens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity | Methylorubrum extorquens |
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