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Literature summary for 4.1.3.22 extracted from

  • Barker, H.A.
    Citramalate lyase of Clostridium tetanomorphum (1967), Arch. Mikrobiol., 59, 4-12.
    View publication on PubMed
Search for more literature for 4.1.3.22

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM Clostridium tetanomorphum
diphosphate
-
 Clostridium tetanomorphum
EDTA
-
 Clostridium tetanomorphum
Fe2+ 1 mM Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 (+)-citramalate
-
 Clostridium tetanomorphum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or an equivalent divalent cation is required Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
no activity in Clostridium kluyveri
-
-
-
no activity in Escherichia coli
-
 ATCC 4157
-
no activity in Pseudomonas sp.
-
-
-
no activity in Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetate + pyruvate
-
 Clostridium tetanomorphum (+)-citramalate
-
 ?
acetate + pyruvate
-
 Clostridium tetanomorphum H1 / ATCC 15920 (+)-citramalate
-
 ?
citramalate
-
 Clostridium tetanomorphum acetate + pyruvate
-
 ?
citramalate
-
 Clostridium tetanomorphum H1 / ATCC 15920 acetate + pyruvate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.5 50 mM potassium phosphate buffer Clostridium tetanomorphum
8 8.2 Tris-HCl buffer Clostridium tetanomorphum

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: 52% of maximal activity, pH 8.0: 77% of maximal activity, in 50 mM potassium phosphate buffer Clostridium tetanomorphum