Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoadipate | competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
2-oxobutanoate | competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
2-oxoglutarate | competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
4-hydroxy-2-oxobutyrate | competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
ATP | competitive | Pseudomonas straminea | |
citrate | competitive | Pseudomonas straminea | |
DL-4-hydroxy-2-oxovalerate | competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | activated by Zn2+ | Pseudomonas straminea | |
0.0074 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | activated by Co2+ | Pseudomonas straminea | |
0.0075 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | activated by Cd2+ | Pseudomonas straminea | |
0.0086 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | activated by Mn2+ | Pseudomonas straminea | |
0.0088 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate | activated by Mg2+ | Pseudomonas straminea | |
0.097 | - |
D-4-carboxy-4-hydroxy-2-oxoadipate | activated by Mg2+ | Pseudomonas straminea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ | Pseudomonas straminea | |
Cd2+ | Km: 0.0034 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
Co2+ | absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ | Pseudomonas straminea | |
Co2+ | Km: 0.0077 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
Mg2+ | Km: 0.154 mM, reaction with D-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
Mg2+ | Km: 0.0323 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
Mg2+ | absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ | Pseudomonas straminea | |
Mn2+ | absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ | Pseudomonas straminea | |
Mn2+ | Km: 0.0059 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea | |
Zn2+ | absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+, Km: 0.0018 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate | Pseudomonas straminea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
6 * 26000, SDS-PAGE | Pseudomonas straminea |
160000 | - |
gel filtration | Pseudomonas straminea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-4-hydroxy-2-oxoadipate | the L-isomer is preferred | Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
4-carboxy-4-hydroxy-2-oxoadipate | no equilibrium due to oxaloacetate beta-decarboxylase activity associated with the enzyme | Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
4-Hydroxy-2-oxoglutarate | the L-isomer is preferred | Pseudomonas straminea | Pyruvate + glyoxylate | - |
? | |
4-hydroxy-4-methyl-2-oxoglutarate | the L-isomer is preferred | Pseudomonas straminea | pyruvate + pyruvate | - |
? | |
D-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
l-4-carboxy-4-hydroxy-2-oxoadipate | - |
Pseudomonas straminea | pyruvate + oxaloacetate | - |
? | |
pyruvate + glyoxylate | - |
Pseudomonas straminea | 4-hydroxy-2-oxoglutarate | - |
? | |
pyruvate + pyruvate | - |
Pseudomonas straminea | 4-hydroxy-4-methyl-2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 26000, SDS-PAGE | Pseudomonas straminea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate, in presence of 0.2 mM phosphate | Pseudomonas straminea |
8.3 | - |
L-4-carboxy-4-hydroxy-2-oxoadipate, in presence of 0.2 mM phosphate | Pseudomonas straminea |
8.4 | - |
DL-4-hydroxy-4-methyl-2-oxoglutarate, in presence of 0.2 mM phosphate | Pseudomonas straminea |
8.9 | - |
oxaloacetate | Pseudomonas straminea |
9.4 | - |
DL-4-hydroxy-2-oxoglutarate, in presence of 0.2 mM oxoglutarate | Pseudomonas straminea |