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Literature summary for 4.1.3.17 extracted from

  • Maruyama, K.
    Purification and properties of 4-hydroxy-4-methyl-2-oxoglutarate aldolase from Pseudomonas ochraceae grown on phthalate (1990), J. Biochem., 108, 327-333.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-oxoadipate competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
2-oxobutanoate competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
2-oxoglutarate competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
4-hydroxy-2-oxobutyrate competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
ATP competitive Pseudomonas straminea
citrate competitive Pseudomonas straminea
DL-4-hydroxy-2-oxovalerate competitive with respect to DL-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
L-4-carboxy-4-hydroxy-2-oxoadipate activated by Zn2+ Pseudomonas straminea
0.0074
-
L-4-carboxy-4-hydroxy-2-oxoadipate activated by Co2+ Pseudomonas straminea
0.0075
-
L-4-carboxy-4-hydroxy-2-oxoadipate activated by Cd2+ Pseudomonas straminea
0.0086
-
L-4-carboxy-4-hydroxy-2-oxoadipate activated by Mn2+ Pseudomonas straminea
0.0088
-
L-4-carboxy-4-hydroxy-2-oxoadipate activated by Mg2+ Pseudomonas straminea
0.097
-
D-4-carboxy-4-hydroxy-2-oxoadipate activated by Mg2+ Pseudomonas straminea

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ Pseudomonas straminea
Cd2+ Km: 0.0034 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
Co2+ absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ Pseudomonas straminea
Co2+ Km: 0.0077 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
Mg2+ Km: 0.154 mM, reaction with D-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
Mg2+ Km: 0.0323 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
Mg2+ absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ Pseudomonas straminea
Mn2+ absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+ Pseudomonas straminea
Mn2+ Km: 0.0059 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea
Zn2+ absolute requirement for divalent metal ion: Mg2+, Mn2+, Co2+, Zn2+ or Cd2+, Km: 0.0018 mM, reaction with L-4-carboxy-4-hydroxy-2-oxoadipate Pseudomonas straminea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
6 * 26000, SDS-PAGE Pseudomonas straminea
160000
-
gel filtration Pseudomonas straminea

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-4-hydroxy-2-oxoadipate the L-isomer is preferred Pseudomonas straminea pyruvate + oxaloacetate
-
?
4-carboxy-4-hydroxy-2-oxoadipate no equilibrium due to oxaloacetate beta-decarboxylase activity associated with the enzyme Pseudomonas straminea pyruvate + oxaloacetate
-
?
4-Hydroxy-2-oxoglutarate the L-isomer is preferred Pseudomonas straminea Pyruvate + glyoxylate
-
?
4-hydroxy-4-methyl-2-oxoglutarate the L-isomer is preferred Pseudomonas straminea pyruvate + pyruvate
-
?
D-4-carboxy-4-hydroxy-2-oxoadipate
-
Pseudomonas straminea pyruvate + oxaloacetate
-
?
l-4-carboxy-4-hydroxy-2-oxoadipate
-
Pseudomonas straminea pyruvate + oxaloacetate
-
?
pyruvate + glyoxylate
-
Pseudomonas straminea 4-hydroxy-2-oxoglutarate
-
?
pyruvate + pyruvate
-
Pseudomonas straminea 4-hydroxy-4-methyl-2-oxoglutarate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 26000, SDS-PAGE Pseudomonas straminea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
L-4-carboxy-4-hydroxy-2-oxoadipate, in presence of 0.2 mM phosphate Pseudomonas straminea
8.3
-
L-4-carboxy-4-hydroxy-2-oxoadipate, in presence of 0.2 mM phosphate Pseudomonas straminea
8.4
-
DL-4-hydroxy-4-methyl-2-oxoglutarate, in presence of 0.2 mM phosphate Pseudomonas straminea
8.9
-
oxaloacetate Pseudomonas straminea
9.4
-
DL-4-hydroxy-2-oxoglutarate, in presence of 0.2 mM oxoglutarate Pseudomonas straminea