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Literature summary for 4.1.3.16 extracted from
- Rat liver 4-hydroxy-2-ketoglutarate aldolase: purification and kinetic characterization (1985), Arch. Biochem. Biophys., 236, 82-97.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxy-2-oxoglutarate | substrate inhibition, Ki: 35 mM | Rattus norvegicus |  |
| beta-Hydroxypyruvate | - |
Rattus norvegicus | |
| Bromopyruvate | Ki: 0.018 mM | Rattus norvegicus | |
| glyoxylate | Ki: 1.5 mM | Rattus norvegicus | |
| Mercaptopyruvate | - |
Rattus norvegicus | |
| additional information | double inhibitor studies | Rattus norvegicus | |
| NaBr | 18% inhibition at 50 mM, only condensation | Rattus norvegicus | |
| NaF | 15% inhibition at 50 mM, only condensation | Rattus norvegicus | |
| oxaloacetate | competitive, Ki: 0.22 mM | Rattus norvegicus | |
| pyruvate | analogs | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | 11% of total activity | Rattus norvegicus | 5737 | - |
| mitochondrion | 89% of total activity | Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| 7.3 | - |
- |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
Bromopyruvate | - |
Rattus norvegicus | |
| 0.22 | - |
oxaloacetate | competitive | Rattus norvegicus | |
| 35 | - |
4-hydroxy-2-oxoglutarate | substrate inhibition | Rattus norvegicus | |
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