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Literature summary for 4.1.3.16 extracted from

  • Dekker, E.E.; Kobes, R.D.; Grady, S.R.
    2-keto-4-Hydroxyglutarate aldolase from bovine liver (1975), Methods Enzymol., 42C, 280-285.
    View publication on PubMed
Search for more literature for 4.1.3.16

Inhibitors

Inhibitors Comment Organism Structure
sulfhydryl-reacting reagents
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bos taurus
0.14
-
 (D)-4-hydroxy-alpha-ketoglutarate
-
 Bos taurus
71
-
 L-4-hydroxy-2-oxoglutarate
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.7
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
5¦C, Tris-HCl buffer, pH 7.4, two weeks, sometimes several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-4-hydroxy-2-oxoglutarate
-
 Bos taurus pyruvate + glyoxylate
-
 ?
additional information Keq: 11 mM Bos taurus ?
-
 ?
Oxaloacetate
-
 Bos taurus CO2 + pyruvate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 2-keto-4-hydroxyglutarate cleavage Bos taurus