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Literature summary for 4.1.3.1 extracted from
- Crystal structure and functional analysis of isocitrate lyases from Magnaporthe oryzae and Fusarium graminearum (2016), J. Struct. Biol., 194, 395-403 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Fusarium graminearum |
| expressed in Escherichia coli BL21(DE3) cells | Pyricularia oryzae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| complexed with Mn2+ and malonate, sitting drop vapor diffusion method, using 2.4 M sodium malonate | Fusarium graminearum |
| ligand-free form and as a complex with Mg2+, glyoxylate, and glycerol, sitting drop vapor diffusion method, using 0.2 M sodium malonate and 20% (w/v) PEG 3350 or 0.2 M potassium sodium tartrate and 10% (v/v) glycerol | Pyricularia oryzae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C215A | inactive | Pyricularia oryzae |
| C215S | inactive | Pyricularia oryzae |
| E206A | inactive | Pyricularia oryzae |
| E405A | inactive | Pyricularia oryzae |
| N433A | inactive | Pyricularia oryzae |
| R252A | inactive | Pyricularia oryzae |
| R252K | inactive | Pyricularia oryzae |
| T524V | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pyricularia oryzae |
| T534A | the mutant shows reduced Km and kcat values compared to the wild type enzyme | Pyricularia oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5287 | - |
isocitrate | wild type enzyme, at pH 7.0 and 37°C | Pyricularia oryzae |  |
| 0.5287 | - |
isocitrate | mutant enzyme T534A, at pH 7.0 and 37°C | Pyricularia oryzae | |
| 0.5433 | - |
isocitrate | mutant enzyme T534V, at pH 7.0 and 37°C | Pyricularia oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Fusarium graminearum | - |
succinate + glyoxylate | - |
? | |
| isocitrate | Pyricularia oryzae | - |
succinate + glyoxylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium graminearum | Q4HYR2 | - |
- |
| Pyricularia oryzae | P0CT06 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Fusarium graminearum |
| Ni-NTA column chromatography and Superdex 200 gel filtration | Pyricularia oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Fusarium graminearum | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Pyricularia oryzae | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | x-ray crystallography | Fusarium graminearum |
| homotetramer | x-ray crystallography | Pyricularia oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Fusarium graminearum |
| ICL | - |
Pyricularia oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.4 | - |
isocitrate | mutant enzyme T534A, at pH 7.0 and 37°C | Pyricularia oryzae | |
| 15.9 | - |
isocitrate | mutant enzyme T534V, at pH 7.0 and 37°C | Pyricularia oryzae | |
| 17.6 | - |
isocitrate | wild type enzyme, at pH 7.0 and 37°C | Pyricularia oryzae | |
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