Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Colwellia psychrerythraea |
Protein Variants | Comment | Organism |
---|---|---|
A214S | increased thermostability and low specific activity at low temperature | Colwellia psychrerythraea |
A231E | more thermolabile than the wild type enzyme | Colwellia psychrerythraea |
A341N | decreased activity compared to the wild type enzyme | Colwellia psychrerythraea |
F333L | very low activity | Colwellia psychrerythraea |
Q47K | activity similar to the wild type enzyme | Colwellia psychrerythraea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58131 | - |
4 * 58131, calculated from amino acid sequence | Colwellia psychrerythraea |
60000 | - |
4 * 60000, gel filtration | Colwellia psychrerythraea |
240000 | - |
gel filtration | Colwellia psychrerythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | A8C5K8 | - |
- |
Colwellia psychrerythraea NRC 1004 | A8C5K8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Colwellia psychrerythraea | succinate + glyoxylate | - |
? | |
isocitrate | - |
Colwellia psychrerythraea NRC 1004 | succinate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 58131, calculated from amino acid sequence | Colwellia psychrerythraea |
homotetramer | 4 * 60000, gel filtration | Colwellia psychrerythraea |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Colwellia psychrerythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Colwellia psychrerythraea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the enzyme is rapidly inactivated at 30°C | Colwellia psychrerythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.3 | - |
isocitrate | mutant enzyme A341N, at 25°C | Colwellia psychrerythraea | |
26.6 | - |
isocitrate | mutant enzyme Q47K, at 25°C | Colwellia psychrerythraea | |
28.5 | - |
isocitrate | wild type enzyme, at 25°C | Colwellia psychrerythraea |