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Literature summary for 4.1.3.1 extracted from

  • Bai, B.; Xie, J.; Yan, J.; Wang, H.; Hu, C.
    A high throughput screening approach to identify isocitrate lyase inhibitors from traditional Chinese medicine sources (2006), Drug Dev. Res., 67, 818-823.
No PubMed abstract available
Search for more literature for 4.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM 109 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
itaconic anhydride
-
 Mycobacterium tuberculosis
XHD-1 extract of Illicium verum Mycobacterium tuberculosis
XHD-2 extract of Zingiber officinale Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.159
-
 isocitrate in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose chromatography Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
4°C, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0, 2 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?

Synonyms

Synonyms Comment Organism
ICL
-
 Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0033
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis itaconic anhydride
0.0182
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis XHD-2
0.0477
-
 in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C Mycobacterium tuberculosis XHD-1