Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.1 extracted from

  • Lu, Z.; Feng, X.; Song, L.; Han, Y.; Kim, A.; Herzberg, O.; Woodson, W.R.; Martin, B.M.; Mariano, P.S.; Dunaway-Mariano, D.
    Diversity of function in the isocitrate lyase enzyme superfamily: the Dianthus caryophyllus petal death protein cleaves alpha-keto and alpha-hydroxycarboxylic acids (2005), Biochemistry, 44, 16365-16376.
    View publication on PubMed
Search for more literature for 4.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expresssion in Escherichia coli Dianthus caryophyllus

Protein Variants

Protein Variants Comment Organism
C144A mutant with no enzyme activity Dianthus caryophyllus
D79A mutant with 1000fold reduced turnover rate Dianthus caryophyllus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C Dianthus caryophyllus
0.029
-
 (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C Dianthus caryophyllus
0.042
-
 (2R,3S)-isocitrate pH 7.5, 25°C Dianthus caryophyllus
0.069
-
 (2R,3S:2S,3R)-2-methyl-isocitrate pH 7.5, 25°C Dianthus caryophyllus
0.098
-
 (2R)-propyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
0.104
-
 (2R,3R:2S,3S)-2-methyl-isocitrate pH 7.5, 25°C Dianthus caryophyllus
0.13
-
 oxalacetate pH 7.5, 25°C Dianthus caryophyllus
0.29
-
 (2R)-2-methyl malate pH 7.5, 25°C Dianthus caryophyllus
0.45
-
 (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
0.53
-
 (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
1.1
-
 (2R)-2-ethyl malate pH 7.5, 25°C Dianthus caryophyllus
8
-
 (2R)-isobutyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activating Dianthus caryophyllus
Fe2+ activating Dianthus caryophyllus
Mg2+ activating oxalacetate hydrolase activity Dianthus caryophyllus
Mn2+ activating oxalacetate hydrolase activity Dianthus caryophyllus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 4 * 35000, SDS-PAGE Dianthus caryophyllus
133000
-
 gel filtration Dianthus caryophyllus

Organism

Organism UniProt Comment Textmining
Dianthus caryophyllus Q05957
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dianthus caryophyllus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-ethyl malate
-
 Dianthus caryophyllus acetate + 2-oxobutanoate
-
 ?
(2R)-2-methyl malate
-
 Dianthus caryophyllus pyruvate + acetate
-
 ?
(2R)-ethyl-(3S)-methyl malate
-
 Dianthus caryophyllus 2-oxobutanoate + propanoate
-
 ?
(2R)-isobutyl-(3S)-methyl malate
-
 Dianthus caryophyllus 4-methyl-2-oxopentanoate + propanoate
-
 ?
(2R)-propyl-(3S)-methyl malate
-
 Dianthus caryophyllus 4-methyl-2-oxopentanoate + propanoate
-
 ?
(2R,3R:2S,3S)-2-methyl-isocitrate
-
 Dianthus caryophyllus succinate + pyruvate
-
 ?
(2R,3S)-2,3-dimethyl malate
-
 Dianthus caryophyllus pyruvate + propanoate
-
 ?
(2R,3S)-isocitrate
-
 Dianthus caryophyllus succinate + glyoxylate
-
 ?
(2R,3S:2S,3R)-2-methyl-isocitrate
-
 Dianthus caryophyllus succinate + pyruvate
-
 ?
(2S)-2-methyl malate kcat below 1x10-5 Dianthus caryophyllus pyruvate + acetate
-
 ?
(R)-malate kcat below 1x10-5 Dianthus caryophyllus glyoxylate + acetate
-
 ?
(S)-malate kcat below 1x10-5 Dianthus caryophyllus glyoxylate + acetate
-
 ?
oxalacetate
-
 Dianthus caryophyllus oxalate + acetate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 35000, SDS-PAGE Dianthus caryophyllus

Synonyms

Synonyms Comment Organism
PDP
-
 Dianthus caryophyllus
petal death protein
-
 Dianthus caryophyllus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 (2R,3S)-2-ethyl-3-methyl malate Dianthus caryophyllus