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Literature summary for 4.1.3.1 extracted from
- Molecular characterization of a bifunctional glyoxylate cycle enzyme, malate synthase/isocitrate lyase, in Euglena gracilis (2005), Comp. Biochem. Physiol. B, 141, 445-452.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | more than 4fold increased enzyme activity in the presence of 150 microM acetyl-CoA | Euglena gracilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
acetyl-CoA | pH 8.0, 30°C | Euglena gracilis | |
| 0.04 | - |
glyoxylate | pH 8.0, 30°C | Euglena gracilis | |
| 7.6 | - |
isocitrate | pH 6.5, 30°C | Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
4 * 110000, SDS-PAGE | Euglena gracilis |
| 420000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | Q8LPA6 | SM-ZK | - |
| Euglena gracilis SM-ZK | Q8LPA6 | SM-ZK | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.636 | - |
isocitrate lyase activity | Euglena gracilis |
| 5.19 | - |
malate synthase activity | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glyoxylate + H2O | - |
Euglena gracilis | malate + CoA | - |
? | |
| acetyl-CoA + glyoxylate + H2O | - |
Euglena gracilis SM-ZK | malate + CoA | - |
? | |
| isocitrate | - |
Euglena gracilis | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Euglena gracilis SM-ZK | succinate + glyoxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 110000, SDS-PAGE | Euglena gracilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EgGCE | bifunctional enzyme: isocitrate lyase and malate synthase | Euglena gracilis |
| ICL | - |
Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
isocitrate lyase | Euglena gracilis |
| 7.5 | 8.5 | malate synthase | Euglena gracilis |
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