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Literature summary for 4.1.3.1 extracted from

  • Honer Zu Bentrup, K.; Miczak, A.; Swenson, D.L.; Russell, D.G.
    Characterization of activity and expression of isocitrate lyase in Mycobacterium avium and Mycobacterium tuberculosis (1999), J. Bacteriol., 181, 7161-7167.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
Icl and AceA are different open reading frames, both with isocitrate lyase activity Mycobacterium tuberculosis

General Stability

General Stability Organism
unstable, loses its activity within a couple of hours on ice, AceA enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
3-nitropropionate
-
 Mycobacterium tuberculosis
Bromopyruvate
-
 Mycobacterium tuberculosis
Itaconate
-
 Mycobacterium tuberculosis
itaconic anhydride
-
 Mycobacterium tuberculosis
malate Icl enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis
oxalate Icl enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis
succinate AceA enzyme, 50% inhibition at 5 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 threo D-(s)-isocitrate Icl enzyme, 37°C Mycobacterium tuberculosis
1.3
-
 threo D-(s)-isocitrate AceA enzyme, Tricine buffer, 37°C, pH 7.5 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Icl enzyme: most effective cation, optimal concentration: 5 mM Mycobacterium tuberculosis
Mn2+ Icl enzyme: can replace Mg2+, yielding 39% of the activity obtained with Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Mycobacterium tuberculosis
-
 succinate + glyoxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
 Icl enzyme Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
-80°C, stable for several months when frozen in liquid nitrogen and stored at -80°C, Icl enzyme Mycobacterium tuberculosis
4°C, after thawing and storage at 4°C the loss of activity after 2 months is minimal in the presence of glycerol and a reducing agent, Icl enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?

Synonyms

Synonyms Comment Organism
AceA
-
 Mycobacterium tuberculosis
ICL
-
 Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 Icl enzyme Mycobacterium tuberculosis
7.5
-
 AceA enzyme Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 3-nitropropionate Icl enzyme, 37°C Mycobacterium tuberculosis
0.11
-
 3-nitropropionate AceA enzyme, 37°C Mycobacterium tuberculosis
0.12
-
 Bromopyruvate Icl enzyme, 37°C Mycobacterium tuberculosis
0.12
-
 Itaconate Icl enzyme, 37°C Mycobacterium tuberculosis
0.19
-
 itaconic anhydride Icl enzyme, 37°C Mycobacterium tuberculosis
0.22
-
 Itaconate AceA enzyme, 37°C Mycobacterium tuberculosis
0.48
-
 itaconic anhydride AceA enzyme, 37°C Mycobacterium tuberculosis
0.71
-
 Bromopyruvate AceA enzyme, 37°C Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis calculated from amino acid sequence
-
 5