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Literature summary for 4.1.3.1 extracted from

  • Munir, E.; Hattori, T.; Shimada, M.
    Purification and characterization of isocitrate lyase from the wood-destroying basidiomycete Fomitopsis palustris grown on glucose (2002), Arch. Biochem. Biophys., 399, 225-231.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 41% increase of activity at 1 mM Fomitopsis palustris
dithiothreitol 65% increase of activity at 1 mM Fomitopsis palustris
glutathione 35% increase of activity at 1 mM Fomitopsis palustris
L-cysteine 45% increase of activity at 1 mM Fomitopsis palustris

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate complete inactivation at 0.001 mM Fomitopsis palustris
iodoacetate isocitrate protects Fomitopsis palustris
Itaconate 89% inhibition at 1 mM Fomitopsis palustris
oxalate 86% inhibition at 10 mM Fomitopsis palustris
oxaloacetate 89% inhibition at 10 mM Fomitopsis palustris
pyruvate 87% inhibition at 10 mM Fomitopsis palustris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.092
-
Mg2+ pH 7 Fomitopsis palustris
1.6
-
DL-isocitrate pH 7 Fomitopsis palustris

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, optimal concentration: 2 mM, Km value: 0.092 at pH 7 Fomitopsis palustris
Mn2+ can replace Mg2+ with 24% of the activity of Mg2+ Fomitopsis palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
3 * 60000, SDS-PAGE Fomitopsis palustris
186000
-
gel filtration Fomitopsis palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Fomitopsis palustris regulatory enzyme playing a crucial role in fungal growth succinate + glyoxylate
-
?

Organism

Organism UniProt Comment Textmining
Fomitopsis palustris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
76fold Fomitopsis palustris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.3
-
-
Fomitopsis palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
Fomitopsis palustris succinate + glyoxylate
-
?
isocitrate regulatory enzyme playing a crucial role in fungal growth Fomitopsis palustris succinate + glyoxylate
-
?

Subunits

Subunits Comment Organism
trimer 3 * 60000, SDS-PAGE Fomitopsis palustris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
DL-isocitrate pH 7 Fomitopsis palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Fomitopsis palustris

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 50% activity at pH 5, 22% activity at pH 8.5, no activity at pH 9 Fomitopsis palustris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.037
-
oxalate pH 7 Fomitopsis palustris
0.068
-
Itaconate pH 7 Fomitopsis palustris