Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Pseudomonas putida | rate-limiting enzyme of the citric acid cycle | succinate + glyoxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
wild-type: KT2442, mutant: KT217, poly(3-hydroxyalkanoate)-overproducing mutant in which aceA gene is knocked out | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
knock-out mutant enzyme after growth on gluconate, crude extract | Pseudomonas putida |
0.003 | - |
knock-out mutant enzyme after growth on gluconate + heptanoate, crude extract | Pseudomonas putida |
0.043 | - |
wild-type enzyme after growth on gluconate, crude extract | Pseudomonas putida |
0.321 | - |
wild-type enzyme after growth on gluconate + heptanoate, crude extract | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Pseudomonas putida | succinate + glyoxylate | - |
? | |
isocitrate | rate-limiting enzyme of the citric acid cycle | Pseudomonas putida | succinate + glyoxylate | - |
? |