Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.1 extracted from

  • Nakamura, K.; Amano, Y.; Nakadate, M.; Kagami, M.
    Purification and properties of isocitrate lyase from Candida brassicae E-17 (1989), J. Ferment. Bioeng., 67, 153-157.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol increase of activity Candida brassicae
cysteine increase of activity Candida brassicae
dithioerythritol increase of activity Candida brassicae
dithiothreitol increase of activity Candida brassicae
glutathione increase of activity Candida brassicae
thiol compounds increase of activity Candida brassicae

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol increase of activity Candida brassicae
glutathione increase of activity Candida brassicae
glycolate
-
Candida brassicae
malonate
-
Candida brassicae
Mn2+ inhibition in presence of Mg2+, activation in absence of Mg2+ Candida brassicae
succinate
-
Candida brassicae
Zn2+
-
Candida brassicae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
DL-isocitrate imidazole-HCl buffer pH 6.85 Candida brassicae
1.532
-
DL-isocitrate sodium phosphate buffer, pH 6.85 Candida brassicae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ Ba2+ less effective activator than Mg2+ Candida brassicae
Mg2+ maximal activity at 8 mM Candida brassicae
Mn2+ Mn2+ less effective activator than Mg2+ Candida brassicae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
290000
-
gel filtration Candida brassicae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Candida brassicae glyoxylate cycle ?
-
?

Organism

Organism UniProt Comment Textmining
Candida brassicae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida brassicae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.01
-
-
Candida brassicae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
Candida brassicae succinate + glyoxylate
-
?
isocitrate glyoxylate cycle Candida brassicae ?
-
?

Subunits

Subunits Comment Organism
tetramer 4*71000, SDS-PAGE Candida brassicae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Candida brassicae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Candida brassicae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 stable Candida brassicae