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Literature summary for 4.1.3.1 extracted from
- Purification and characterization of Acinetobacter calcoaceticus isocitrate lyase (1991), J. Bacteriol., 173, 6844-6848.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Acinetobacter calcoaceticus |  |
| Ca2+ | - |
Acinetobacter calcoaceticus | |
| glycolate | - |
Acinetobacter calcoaceticus | |
| Hydroxymalonate | - |
Acinetobacter calcoaceticus | |
| malonate | - |
Acinetobacter calcoaceticus | |
| methylenesuccinate | - |
Acinetobacter calcoaceticus | |
| oxalate | - |
Acinetobacter calcoaceticus | |
| Sr2+ | - |
Acinetobacter calcoaceticus | |
| Tartrate | - |
Acinetobacter calcoaceticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
threo-Ds-isocitrate | - |
Acinetobacter calcoaceticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no activity in absence of exogenous cation | Acinetobacter calcoaceticus | |
| Co2+ | Co2+ 12% as effective as Mg2+ | Acinetobacter calcoaceticus | |
| Fe2+ | no activity in absence of exogenous cation, Fe2+ 7% as effective as Mg2+ | Acinetobacter calcoaceticus | |
| Mg2+ | increase of activity | Acinetobacter calcoaceticus | |
| Mn2+ | no activity in absence of exogenous cation | Acinetobacter calcoaceticus | |
| Mn2+ | Mn2+ 14% as effective as Mg2+ | Acinetobacter calcoaceticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Acinetobacter calcoaceticus |
| 250000 | - |
gel filtration | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Acinetobacter calcoaceticus | glyoxylate cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter calcoaceticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
- |
Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-isocitrate | - |
Acinetobacter calcoaceticus | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Acinetobacter calcoaceticus | succinate + glyoxylate | - |
? | |
| isocitrate | glyoxylate cycle | Acinetobacter calcoaceticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 64000, SDS-PAGE | Acinetobacter calcoaceticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Acinetobacter calcoaceticus |
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