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Literature summary for 4.1.3.1 extracted from
- Isocitrate lyase from Neurospora crassa. I. Purification, kinetic mechanism, and interaction with inhibitors (1974), Biochim. Biophys. Acta, 364, 327-340.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thiol compounds | increase of activity | Neurospora crassa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | - |
Neurospora crassa | |
| Cl- | - |
Vogesella indigofera | |
| fructose-1,6-bisphosphate | - |
Neurospora crassa | |
| fumarate | - |
Neurospora crassa | |
| fumarate | - |
Vogesella indigofera | |
| glyoxylate | - |
Neurospora crassa | |
| HPO42- | - |
Neurospora crassa | |
| HPO42- | - |
Vogesella indigofera | |
| malate | - |
Neurospora crassa | |
| malate | - |
Vogesella indigofera | |
| NO3- | - |
Neurospora crassa | |
| NO3- | - |
Vogesella indigofera | |
| phosphoenolpyruvate | - |
Neurospora crassa | |
| SO42- | - |
Neurospora crassa | |
| SO42- | - |
Vogesella indigofera | |
| succinate | - |
Neurospora crassa | |
| succinate | - |
Vogesella indigofera | |
| threo-DL-homoisocitrate | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
glyoxylate | - |
Neurospora crassa | |
| 0.044 | - |
succinate | - |
Neurospora crassa | |
| 0.05 | - |
threo-Ds-isocitrate | - |
Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | increase of activity | Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Neurospora crassa | glyoxylate cycle | ? | - |
? | |
| isocitrate | Vogesella indigofera | glyoxylate cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
| Vogesella indigofera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 6 months, stable | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-isocitrate | - |
Neurospora crassa | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Neurospora crassa | succinate + glyoxylate | - |
r | |
| isocitrate | - |
Vogesella indigofera | succinate + glyoxylate | - |
r | |
| isocitrate | glyoxylate cycle | Neurospora crassa | ? | - |
? | |
| isocitrate | glyoxylate cycle | Vogesella indigofera | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Neurospora crassa |
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