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Literature summary for 4.1.3.1 extracted from

  • Reiss, U.; Rothstein, M.
    Isocitrate lyase from the free-living nematode, Tubatrix aceti: purification and properties (1974), Biochemistry, 13, 1796-1800.
    View publication on PubMed
Search for more literature for 4.1.3.1

Activating Compound

Activating Compound Comment Organism Structure
cysteine increase of activity Turbatrix aceti
sulfhydryl compounds increase of activity Turbatrix aceti

Inhibitors

Inhibitors Comment Organism Structure
malonate
-
 Turbatrix aceti
p-hydroxymercuribenzoate complete inhibition at 0.016 mM Turbatrix aceti
sulfhydryl compounds increase of activity Turbatrix aceti
Tartrate
-
 Turbatrix aceti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 Ds-isocitrate
-
 Turbatrix aceti

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ increase of activity Turbatrix aceti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
123000
-
 4 * 123000, SDS-PAGE Turbatrix aceti
480000
-
 gel filtration Turbatrix aceti

Organism

Organism UniProt Comment Textmining
Turbatrix aceti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Turbatrix aceti

Storage Stability

Storage Stability Organism
-20°C, 4 weeks, 25-30% loss of activity Turbatrix aceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Turbatrix aceti succinate + glyoxylate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 123000, SDS-PAGE Turbatrix aceti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
800
-
 isocitrate
-
 Turbatrix aceti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Turbatrix aceti