Protein Variants | Comment | Organism |
---|---|---|
D60N | site-directed mutagenesis, substitution of asparagine for aspartic acid in the alphaAsp60Asn variant. alphaAsp60 has an essential role in stabilizing charge development on the IGP indole ring during C-C bond scission | Salmonella enterica subsp. enterica serovar Typhimurium |
T183V | site-directed mutagenesis, substitution of valine for threonine in the alphaThr183Val variant, resulting in impaired catalysis at the alpha-site and loss of allosteric communication | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydroxy-3-[(2-hydroxyphenyl)amino] propyl phosphate | competetive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
2-([[4-(trifluoromethoxy)phenyl]sulfonyl]amino) ethyl phosphate | competetive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
3-(1H-indol-3-yl)propyl phosphate | competetive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
3-(2,3-dihydro-1H-indol-3-yl)glycerol phosphate | competetive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
3-acetyl-L-aspartic acid | inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen | Salmonella enterica subsp. enterica serovar Typhimurium | |
4-[(2-aminophenyl)sulfonyl]butyl phosphate | competetive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
indole 3-acetylglycine | inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-glycerol phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | indole + D-glyceraldehyde 3-phosphate | - |
? |