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Literature summary for 4.1.2.8 extracted from

  • Kriechbaumer, V.; Weigang, L.; Fiesselmann, A.; Letzel, T.; Frey, M.; Gierl, A.; Glawischnig, E.
    Characterisation of the tryptophan synthase alpha subunit in maize (2008), BMC Plant Biol., 8, 44.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.8

Cloned(Commentary)

Cloned (Comment) Organism
into the vectorS pET28a, pEZS-NL and pPCVE35E Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.458
-
 (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate
-
 Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Zea mays 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate Zea mays
-
 indole + D-glyceraldehyde 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays B2Y0K4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein by His-tag affinity purification via Ni-NTA agarose, plant protein by anion exchange and gel permeation chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate
-
 Zea mays indole + D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
tryptophan synthase alpha subunit
-
 Zea mays
ZmTSA
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 tryptophan synthase assay Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate
-
 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 tryptophan synthase assay Zea mays