Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxypyruvate | - |
Saccharolobus solfataricus | |
additional information | no decrease in activity in the presence of 50 mM EDTA | Saccharolobus solfataricus | |
NaBH4 | 69% inactivation in the presence of NaBH% and pyruvate, complete inactivation when the reaction time is increased to 20 min | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is unaffected by the presence of 0.1 mM ZnCl2 | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32980 | - |
4 * 32980, calculated from sequence | Saccharolobus solfataricus |
133000 | - |
equilibrium centrifugation analysis | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | O54288 | - |
- |
Saccharolobus solfataricus DSM 1616 | O54288 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.1 U/mg, pH 6.0, 70°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde | no activity could be detected with D-glyceraldehyde 3-phosphate as substrate in place of glyceraldehyde or with the pyruvate analogues beta-hydroxypyruvate and alpha-ketobutyrate. Studies by other groups reveal activity with D-glyceraldehyde 3-phosphate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate | - |
r | |
pyruvate + D-glyceraldehyde | no activity could be detected with D-glyceraldehyde 3-phosphate as substrate in place of glyceraldehyde or with the pyruvate analogues beta-hydroxypyruvate and alpha-ketobutyrate. Studies by other groups reveal activity with D-glyceraldehyde 3-phosphate | Saccharolobus solfataricus DSM 1616 | 2-dehydro-3-deoxy-D-gluconate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32980, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
KDG-aldolase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
95 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | 70°C: about 40% of maximal activity, 100°C: about 95% of maximal activity | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 7.8 h | Saccharolobus solfataricus |
100 | - |
half-life: 2.5 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Hydroxypyruvate | pH 6.0, 70°C | Saccharolobus solfataricus |