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Literature summary for 4.1.2.53 extracted from

  • Rea, D.; Hovington, R.; Rakus, J.F.; Gerlt, J.A.; Fueloep, V.; Bugg, T.D.; Roper, D.I.
    Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12 (2008), Biochemistry, 47, 9955-9965.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 4-hydroxy-2-oxohexanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli
0.078
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.1
-
 4-hydroxy-2-oxopentanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli
0.1
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Ni2+-activated Escherichia coli
0.14
-
 2-dehydro-3-deoxy-L-mannonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.15
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.8
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C, Mg2+-activated Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ metal-dependent enzyme, Mg2+ activates Escherichia coli
Ni2+ metal-dependent enzyme, Ni2+ activates. Compared to Mg2+ the Ni2+-activated enzyme has a higher turnover, resulting in an elevated substrate specificity constant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P76469
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-lyxonate
-
 Escherichia coli ?
-
 ?
2-dehydro-3-deoxy-L-mannonate
-
 Escherichia coli ?
-
 ?
2-dehydro-3-deoxy-L-rhamnonate 2-dehydro-3-deoxy-L-rhamnonate i.e. 3,6-dideoxy-L-erythro-hex-2-ulosonate Escherichia coli pyruvate + (R)-lactaldehyde
-
 ?
4-hydroxy-2-oxoheptane-1,7-dioate
-
 Escherichia coli ?
-
 ?
4-hydroxy-2-oxohexanoate
-
 Escherichia coli ?
-
 ?
4-hydroxy-2-oxopentanoate
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
YfaU
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.3
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.4
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
0.54
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Mg2+-activated Escherichia coli
299
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Ni2+-activated Escherichia coli
396
-
 4-hydroxy-2-oxopentanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli
447
-
 4-hydroxy-2-oxohexanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 2-dehydro-3-deoxy-L-mannonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
3.6
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Mg2+-activated Escherichia coli
3.8
-
 2-dehydro-3-deoxy-L-lyxonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
5.1
-
 2-dehydro-3-deoxy-L-rhamnonate pH 8.0, 25°C, Mg2+-activated Escherichia coli
2700
-
 4-hydroxy-2-oxoheptane-1,7-dioate pH 8.0, 25°C, Ni2+-activated Escherichia coli
3900
-
 4-hydroxy-2-oxopentanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli
9900
-
 4-hydroxy-2-oxohexanoate pH 8.0, 25°C, 25°C, Ni2+-activated Escherichia coli