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Literature summary for 4.1.2.53 extracted from

  • Watanabe, S.; Saimura, M.; Makino, K.
    Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism (2008), J. Biol. Chem., 283, 20372-20382.
    View publication on PubMed
Search for more literature for 4.1.2.53

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Azotobacter vinelandii
expressed in Escherichia coli DH5alpha cells Scheffersomyces stipitis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition in the presence of Ca2+ Azotobacter vinelandii
Co2+ about 75% residual activity in the presence of Co2+ Azotobacter vinelandii
Cu2+ complete inhibition in the presence of Cu2+ Azotobacter vinelandii
Fe2+ complete inhibition in the presence of Fe2+ Azotobacter vinelandii
Mg2+ about 25% residual activity in the presence of Mg2+ Azotobacter vinelandii
Ni2+ about 10% residual activity in the presence of Ni2+ Azotobacter vinelandii
Zn2+ about 80% residual activity in the presence of Zn2+ Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.915
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii
1.37
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 240% activity in the presence of Co2+ Scheffersomyces stipitis
Mg2+ about 150% activity in the presence of Co2+ Scheffersomyces stipitis
Mg2+ more than 250% activity in the presence of Mg2+, 5 mM MgCl2 used in assay conditions Scheffersomyces stipitis
additional information not stimulated by Mg2+, Co2+, Ni2+, Zn2+, Fe2+, Cu2+, and Ca2+ Azotobacter vinelandii
additional information not stimulated by Zn2+, Fe2+, Cu2+, and Ca2+ Scheffersomyces stipitis
Ni2+ about 140% activity in the presence of Ni2+ Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii preferred substrate pyruvate + (R)-lactaldehyde
-
 ?
2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis preferred substrate pyruvate + (R)-lactaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Scheffersomyces stipitis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Azotobacter vinelandii
Ni-NTA column chromatography Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-3-deoxy-L-mannonate 13.3% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis ?
-
 ?
2-dehydro-3-deoxy-L-mannonate 6.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii ?
-
 ?
2-dehydro-3-deoxy-L-rhamnonate preferred substrate Azotobacter vinelandii pyruvate + (R)-lactaldehyde
-
 ?
2-dehydro-3-deoxy-L-rhamnonate preferred substrate Scheffersomyces stipitis pyruvate + (R)-lactaldehyde
-
 ?
2-dehydro-3-deoxy-L-xylonate 54.7% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis ?
-
 ?
2-dehydro-3-deoxy-L-xylonate 7.6% activity compared to 2-dehydro-3-deoxy-L-rhamnonate Azotobacter vinelandii ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 35000, SDS-PAGE Azotobacter vinelandii
? x * about 35000, SDS-PAGE Scheffersomyces stipitis

Synonyms

Synonyms Comment Organism
L-KDR aldolase
-
 Azotobacter vinelandii
L-KDR aldolase
-
 Scheffersomyces stipitis
LRA4
-
 Azotobacter vinelandii
LRA4
-
 Scheffersomyces stipitis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49.3
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis
54.7
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
36.2
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Scheffersomyces stipitis
60.2
-
 2-dehydro-3-deoxy-L-rhamnonate in 50 mM sodium phosphate (pH 7.0), 5 mM Mn2+, temperature not specified in the publication Azotobacter vinelandii