Any feedback?
Literature summary for 4.1.2.5 extracted from
- Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary alpha-amino acids (2015), Appl. Microbiol. Biotechnol., 99, 9651-9661 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Aeromonas veronii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii |  |
| 2.76 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 10.9 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeromonas veronii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aeromonas veronii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-methyl-beta-phenylserine | - |
Aeromonas veronii | ? | - |
? | |
| D-alanine + 3-nitrobenzaldehyde | 23% conversion | Aeromonas veronii | beta-(3-nitrophenyl)-alpha-methyl-serine | - |
? | |
| D-alanine + benzaldehyde | 10% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-methyl-3-phenylpropanoic acid | - |
? | |
| D-alanine + hexanal | 4% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-methyloctanoic acid | - |
? | |
| D-beta-phenylserine | - |
Aeromonas veronii | glycine + benzaldehyde | - |
? | |
| D-serine + 3-nitrobenzaldehyde | 2% conversion | Aeromonas veronii | beta-(3-nitrophenyl)-alpha-hydroxymethyl-serine | - |
? | |
| D-serine + benzaldehyde | 4% conversion | Aeromonas veronii | 2-amino-3-hydroxy-2-hydroxymethyl-3-phenylpropanoic acid | - |
? | |
| D-threonine | - |
Aeromonas veronii | glycine + acetaldehyde | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LTA | - |
Aeromonas veronii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
| 0.54 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 18.3 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | pyridoxal 5'-phosphate-dependent enzyme | Aeromonas veronii | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0027 | - |
D-threonine | pH 8.0, temperature not specified in the publication | Aeromonas veronii | |
| 0.195 | - |
alpha-methyl-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
| 9.2 | - |
D-beta-phenylserine | 25°C, pH 8.0 | Aeromonas veronii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
