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Literature summary for 4.1.2.49 extracted from

  • Liu, J.Q.; Dairi, T.; Kataoka, M.; Shimizu, S.; Yamada, H.
    L-allo-Threonine aldolase from Aeromonas jandaei DK-39: gene cloning, nucleotide sequencing, and identification of the pyridoxal 5'-phosphate-binding lysine residue by site-directed mutagenesis (1997), J. Bacteriol., 179, 3555-3560.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.49

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in recombinant Escherichia coli cells Aeromonas jandaei

Protein Variants

Protein Variants Comment Organism
K199A catalytically inactive mutant enzyme Aeromonas jandaei
K224A mutant enzyme shows properties similar to the wild type enzyme Aeromonas jandaei
K51A mutant enzyme shows properties similar to the wild type enzyme Aeromonas jandaei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K51A Aeromonas jandaei
0.37
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
0.38
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K224A Aeromonas jandaei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36294
-
 4 * 36294, calculated from sequence Aeromonas jandaei

Organism

Organism UniProt Comment Textmining
Aeromonas jandaei O07051
-
-
Aeromonas jandaei DK-39 O07051
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeromonas jandaei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.4
-
 pH 8.0, 30°C Aeromonas jandaei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine
-
 Aeromonas jandaei glycine + acetaldehyde
-
 r
L-allo-threonine
-
 Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 36294, calculated from sequence Aeromonas jandaei

Synonyms

Synonyms Comment Organism
L-allo-TA
-
 Aeromonas jandaei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aeromonas jandaei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K51A Aeromonas jandaei
3
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
3
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K224A Aeromonas jandaei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Aeromonas jandaei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Lys199 probably functions as an essential catalytic residue forming an internal Schiff base with pyridoxal 5'-phosphate of the enzyme to catalyze the reversible aldol reaction Aeromonas jandaei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.9
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K224A Aeromonas jandaei
8.1
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme K51A Aeromonas jandaei
8.1
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei