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Literature summary for 4.1.2.48 extracted from

  • Giger, L.; Toscano, M.; Bouzon, M.; Marlière, P.; Hilvert, D.
    A novel genetic selection system for PLP-dependent threonine aldolases (2012), Tetrahedron, 68, 7549-7557.
No PubMed abstract available
Search for more literature for 4.1.2.48

Cloned(Commentary)

Cloned (Comment) Organism
-
 Caulobacter vibrioides

Protein Variants

Protein Variants Comment Organism
D176E 500fold decrease in catalytic efficiency Caulobacter vibrioides
D95C less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
D95H/E96G less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
D95L less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
D95M less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
D95N/E96S less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
D95W less than 2% of catalytic efficiency of wild-type Caulobacter vibrioides
D95Y less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
D95Y/E96T less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
1 2 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
1 3 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
2.5
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
2.7
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
2.9
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.4
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
7.1
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
11
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
13.6
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
32
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
44
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
44
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
50
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
53
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
63
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides Q9A3V8
-
-
Caulobacter vibrioides CB15 Q9A3V8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-allo-threonine
-
 Caulobacter vibrioides glycine + acetaldehyde
-
 ?
L-allo-threonine
-
 Caulobacter vibrioides CB15 glycine + acetaldehyde
-
 ?
L-threonine
-
 Caulobacter vibrioides glycine + acetaldehyde
-
 ?
L-threonine
-
 Caulobacter vibrioides CB15 glycine + acetaldehyde
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
0.2
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
0.6
-
 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
3
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
3
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
3.3
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
3.5
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
3.6
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
3.8
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
8.6
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
9.6
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
19
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
19
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
20
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
20
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
22
-
 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0016
-
 L-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
0.0063
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
0.028
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
0.046
-
 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
0.056
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
0.068
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
0.072
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
0.072
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
0.218
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
0.24
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
0.68
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
1.7
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
1.8
-
 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
3
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
7.4
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
7.6
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
29
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides