Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.48 extracted from

  • Zhao, G.H.; Li, H.; Liu, W.; Zhang, W.G.; Zhang, F.; Liu, Q.; Jiao, Q.C.
    Preparation of optically active beta-hydroxy-alpha-amino acid by immobilized Escherichia coli cells with serine hydroxymethyl transferase activity (2011), Amino Acids, 40, 215-220.
    View publication on PubMed

Application

Application Comment Organism
synthesis synthesis of optically active beta-hydroxy-alpha-amino acids by immobilized Escherichia coli cells expressing the enzyme. The immobilized cells can be continuously used 10 times, yielding an average conversion rate of 60.4% Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
L-allo-threonine pH 7.0, 37°C Escherichia coli
19.2
-
DL-threo-phenylserine pH 7.0, 37°C Escherichia coli
21.5
-
DL-threo-(3-nitrophenyl)serine pH 7.0, 37°C Escherichia coli
23.6
-
DL-threo-(3-methylsulfonylphenyl)serine pH 7.0, 37°C Escherichia coli
43.1
-
L-threonine pH 7.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A825 serine hydroxymethyl transferase with threonine aldolase activity; MG1655
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-threo-(3-methylsulfonylphenyl)serine 121% of the activity with L-threonine Escherichia coli glycine + 3-methylsulfonylbenzaldehyde
-
?
DL-threo-(3-nitrophenyl)serine 143% of the activity with L-threonine Escherichia coli glycine + 3-nitrobenzaldehyde
-
?
DL-threo-phenylserine 180% of the activity with L-threonine Escherichia coli glycine + benzaldehyde
-
?
L-allo-threonine 291% of the activity with L-threonine Escherichia coli glycine + acetaldehyde
-
?
L-threonine
-
Escherichia coli glycine + acetaldehyde
-
?

Synonyms

Synonyms Comment Organism
GlyA
-
Escherichia coli