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Literature summary for 4.1.2.48 extracted from
- Synthesis of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) with thermostabilized low-specific L-threonine aldolase from Streptomyces coelicolor A3(2) (2007), J. Microbiol. Biotechnol., 17, 721-727.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain JM109 | Streptomyces coelicolor |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A169T | stability-enhanced mutant, half life at 63°C is 3.7 min | Streptomyces coelicolor |
| D104N | stability-enhanced mutant, half life at 63°C is 5.8 min | Streptomyces coelicolor |
| F18I | stability-enhanced mutant, half life at 63°C is 5.0 min, the specific activity is decreased by 45% compared to the wild type enzyme | Streptomyces coelicolor |
| H177Y | stability-enhanced mutant, half life at 63°C is 14.6 min | Streptomyces coelicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000002 | - |
L-phenylserine | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor |  |
| 0.0000026 | - |
L-phenylserine | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.00016 | - |
L-Thr | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.00018 | - |
L-Thr | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.0002 | - |
L-threo-3,4-dihydroxyphenylserine | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.00023 | - |
L-threo-3,4-dihydroxyphenylserine | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
subunit, SDS-PAGE | Streptomyces coelicolor |
| 37500 | - |
4 * 37500, gel filtration | Streptomyces coelicolor |
| 150000 | - |
gel filtration | Streptomyces coelicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | - |
- |
- |
| Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hiprep 16/10 DEAE FF column chromatography, Super Sepharose column chromatography, and HiLoad 16/10 Phenyl Sepharose HP column chromatography | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + 3,4-dihydroxybenzaldehyde | L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated | Streptomyces coelicolor | L-threo-3,4-dihydroxyphenylserine | - |
? | |
| glycine + 3,4-dihydroxybenzaldehyde | L-threo-3,4-dihydroxyphenylserine synthesis activity is dramatically decreased when the condensation reaction is repeated | Streptomyces coelicolor A3(2) | L-threo-3,4-dihydroxyphenylserine | - |
? | |
| L-phenylserine | - |
Streptomyces coelicolor | ? | - |
? | |
| L-phenylserine | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
| L-Thr | - |
Streptomyces coelicolor | glycine + acetaldehyde | - |
? | |
| L-Thr | - |
Streptomyces coelicolor A3(2) | glycine + acetaldehyde | - |
? | |
| L-threo-3,4-dihydroxyphenylserine | - |
Streptomyces coelicolor | glycine + 3,4-dihydroxybenzaldehyde | - |
? | |
| L-threo-3,4-dihydroxyphenylserine | - |
Streptomyces coelicolor A3(2) | glycine + 3,4-dihydroxybenzaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 37500, gel filtration | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-TA | - |
Streptomyces coelicolor |
| L-threonine aldolase | - |
Streptomyces coelicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Streptomyces coelicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the residual L-TA activity after a heat treatment for 20 min at 60°C is 10.6% | Streptomyces coelicolor |
| 63 | - |
the half-life of the wild type L-TA at 63°C is 1.3 min | Streptomyces coelicolor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000107 | - |
L-phenylserine | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.000113 | - |
L-phenylserine | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.00022 | - |
L-threo-3,4-dihydroxyphenylserine | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.00023 | - |
L-threo-3,4-dihydroxyphenylserine | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.0178 | - |
L-Thr | mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
| 0.0195 | - |
L-Thr | recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) | Streptomyces coelicolor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | recombinant enzyme | Streptomyces coelicolor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | activates | Streptomyces coelicolor | |
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