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Literature summary for 4.1.2.47 extracted from

  • Bauer, M.; Geyer, R.; Boy, M.; Griegl, G.; Steiner, W.
    Stability of the enzyme (S)-hydroxynitrile lyase from Hevea brasiliensis (1998), J. Mol. Catal. B, 5, 343-347.
No PubMed abstract available

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis P52704
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelonitrile
-
Hevea brasiliensis cyanide + benzaldehyde
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Hevea brasiliensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
half-life: 1755 min in phosphate buffer, 1379 min in glutamate buffer, 2315 min in citrate buffer Hevea brasiliensis
40
-
half-life: 690 min in phosphate buffer, 204 min in glutamate buffer, 322 min in citrate buffer Hevea brasiliensis
50
-
half-life: 342 min in phosphate buffer, 80 min in glutamate buffer, 115 min in citrate buffer Hevea brasiliensis
60
-
half-life: 56 min in phosphate buffer, 42 min in glutamate buffer, 29 min in citrate buffer Hevea brasiliensis
70
-
half-life: 10 min in phosphate buffer, 15 min in glutamate buffer, 7 min in citrate buffer Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Hevea brasiliensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
enzyme is inactivated very fast. Stability decreases with higher buffer concentrations at pH 3.5 in all three buffer systems (phosphate buffer, glutamate buffer, citrate buffer) Hevea brasiliensis
6.5
-
very stable above. At pH 6.5 stability increases with increasing buffer concentration in all three systems (phosphate buffer, glutamate buffer, citrate buffer). The enzyme is most stable in phosphate buffer whereas for glutamate buffer the relative increase of half-life with concentration is more pronounced Hevea brasiliensis