Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | P52704 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelonitrile | - |
Hevea brasiliensis | cyanide + benzaldehyde | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hevea brasiliensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life: 1755 min in phosphate buffer, 1379 min in glutamate buffer, 2315 min in citrate buffer | Hevea brasiliensis |
40 | - |
half-life: 690 min in phosphate buffer, 204 min in glutamate buffer, 322 min in citrate buffer | Hevea brasiliensis |
50 | - |
half-life: 342 min in phosphate buffer, 80 min in glutamate buffer, 115 min in citrate buffer | Hevea brasiliensis |
60 | - |
half-life: 56 min in phosphate buffer, 42 min in glutamate buffer, 29 min in citrate buffer | Hevea brasiliensis |
70 | - |
half-life: 10 min in phosphate buffer, 15 min in glutamate buffer, 7 min in citrate buffer | Hevea brasiliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Hevea brasiliensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
enzyme is inactivated very fast. Stability decreases with higher buffer concentrations at pH 3.5 in all three buffer systems (phosphate buffer, glutamate buffer, citrate buffer) | Hevea brasiliensis |
6.5 | - |
very stable above. At pH 6.5 stability increases with increasing buffer concentration in all three systems (phosphate buffer, glutamate buffer, citrate buffer). The enzyme is most stable in phosphate buffer whereas for glutamate buffer the relative increase of half-life with concentration is more pronounced | Hevea brasiliensis |