Crystallization (Comment) | Organism |
---|---|
vapor diffusion hanging drop method. X-ray crystal structures (at 1.54 and 1.76 A resolution) of HbHNL complexes with two chiral substrates (S)-mandelonitrile and (2S)-2,3-dimethyl-2-hydroxybutyronitrile by soaking and rapid freeze quenching techniques. Only the S-enantiomers of the two substrates are observed in the active site | Hevea brasiliensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-2-methylpropanenitrile | Hevea brasiliensis | - |
cyanide + acetone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | P52704 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2,3-dimethyl-2-hydroxybutyronitrile | binding mode of the chiral substrates is identical to that observed for the biological substrate 2-hydroxy-2-methylpropanenitrile (i.e. acetone cyanohydrin). Three-point binding mode of the substrates: hydrophobic pocket, hydrogen bonds between the hydroxyl group and Ser80 and Thr11, electrostatic interaction of the cyano group with Lys236 | Hevea brasiliensis | ? | - |
? | |
(S)-mandelonitrile | binding mode of the chiral substrate is identical to that observed for the biological substrate acetone cyanohydrin. Three-point binding mode of the substrates: hydrophobic pocket, hydrogen bonds between the hydroxyl group and Ser80 and Thr11, electrostatic interaction of the cyano group with Lys236 | Hevea brasiliensis | cyanide + benzaldehyde | - |
? | |
2-hydroxy-2-methylpropanenitrile | - |
Hevea brasiliensis | cyanide + acetone | - |
? | |
2-hydroxy-2-methylpropanenitrile | i.e. acetone cyanohydrin, three-point binding mode of the substrates: hydrophobic pocket, hydrogen bonds between the hydroxyl group and Ser80 and Thr11, electrostatic interaction of the cyano group with Lys236 | Hevea brasiliensis | cyanide + acetone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HbHNL | - |
Hevea brasiliensis |