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Literature summary for 4.1.2.47 extracted from

  • Krammer, B.; Rumbold, K.; Tschemmernegg, M.; Poechlauer, P.; Schwab, H.
    A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening (2007), J. Biotechnol., 129, 151-161.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis P52704 recombinantly expressed in Escherichia coli
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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screening assay for hydroxynitrile lyases and its application in high-throughput screening of Escherichia coli mutant libraries, semi-quantitative test where the rate of colour formation corresponds to the particular enzyme activity of the sample Hevea brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-hydroxy(3-phenoxyphenyl)ethanenitrile
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Hevea brasiliensis cyanide + 3-phenoxybenzaldehyde
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r
(S)-mandelonitrile
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Hevea brasiliensis cyanide + benzaldehyde
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?

Synonyms

Synonyms Comment Organism
HbHNL
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Hevea brasiliensis