Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.2.47 extracted from

  • Wajant, H.; Foerster, S.
    Purification and characterization of hydroxynitrile lyase from Hevea brasiliensis (1996), Plant Sci., 115, 25-31.
No PubMed abstract available
Search for more literature for 4.1.2.47

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate 3 MM, 93% inhibition Hevea brasiliensis
diisopropyl fluorophosphate 3 mM, 96% inhibition Hevea brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
115
-
 acetone cyanohydrin pH 5.4 Hevea brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 3 * 30000, SDS-PAGE Hevea brasiliensis
100000 105000 gel filtration Hevea brasiliensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetone cyanohydrin Hevea brasiliensis
-
 cyanide + acetone
-
 ?

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis P52704
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hevea brasiliensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
86.9
-
-
 Hevea brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetone cyanohydrin
-
 Hevea brasiliensis cyanide + acetone
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 30000, SDS-PAGE Hevea brasiliensis

Synonyms

Synonyms Comment Organism
HbHNL
-
 Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3 5.7 substrate: acetone cyanohydrin Hevea brasiliensis