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Literature summary for 4.1.2.46 extracted from

  • Cabirol, F.L.; Tan, P.L.; Tay, B.; Cheng, S.; Hanefeld, U.; Sheldon, R.A.
    Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst (2008), Adv. Synth. Catal., 350, 2329-2338.
No PubMed abstract available
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Activating Compound

Activating Compound Comment Organism Structure
additional information molecular imprinting using 2-butanone as additive in the immobilization process improves the synthetic activity of the biocatalyst Linum usitatissimum

Organism

Organism UniProt Comment Textmining
Linum usitatissimum
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-
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Linum usitatissimum P93243
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 development of an immobilized form of the hydroxynitrile lyase as crosslinked enzyme aggregate (CLEA) with high specific activity (303.5 U/g) and recovery (33%), 180.5 U/g LuCLEA (crosslinked enzyme aggregate) (using sat. (NH4)2SO4), 9.9 U/g LuEA (Enzyme aggregate of LuHNL) (using sat. (NH4)2SO4), 110.9 U/g LuCLEA (using tert-butanol), 221.9 U/g LuEA (using tert-butanol) Linum usitatissimum
additional information
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 development of an immobilized form of the hydroxynitrile lyase as crosslinked enzyme aggregate with high specific activity (303.5 U/g) and recovery Linum usitatissimum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanide + butan-2-one
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 Linum usitatissimum (2R)-2-hydroxy-2-methylbutanenitrile
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 ?
cyanide + butan-2-one reaction with an immobilized form of the hydroxynitrile lyase as crosslinked enzyme aggregate with high specific activity and recovery on a preparative scale Linum usitatissimum (2R)-2-hydroxy-2-methylbutanenitrile
-
 ?

Synonyms

Synonyms Comment Organism
Hydroxynitrile lyase
-
 Linum usitatissimum
LuHNL
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 Linum usitatissimum