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Literature summary for 4.1.2.43 extracted from

  • Sahm, H.; Schuette, H.; Kula, M.R.
    3-Hexulose-phosphate synthase from Methylomonas M15 (1982), Methods Enzymol., 90, 319-323.
No PubMed abstract available
Search for more literature for 4.1.2.43

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 formaldehyde in 300 mM Tris-HCI buffer (pH 7.5), at 30°C Methylomonas sp.
1.6
-
 D-ribulose 5-phosphate in 300 mM Tris-HCI buffer (pH 7.5), at 30°C Methylomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000, SDS-PAGE Methylomonas sp.
40000
-
 gel filtration Methylomonas sp.
43000
-
 sedimentation equilibrium method Methylomonas sp.

Organism

Organism UniProt Comment Textmining
Methylomonas sp.
-
-
-
Methylomonas sp. M15 / DSM 580
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, and Sephadex G-75 gel filtration Methylomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.5
-
 crude extract, at 30°C Methylomonas sp.
66.5
-
 after 14.8fold purification, at 30°C Methylomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. D-arabino-hex-3-ulose 6-phosphate
-
 ?
D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. M15 / DSM 580 D-arabino-hex-3-ulose 6-phosphate
-
 ?
additional information no detectable activity with D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 1-phosphate, D-glucose 6-phosphate, lithium hydroxypyruvate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, D-ribose 5-phosphate, and D-xylose 5-phosphate Methylomonas sp. ?
-
 ?
additional information no detectable activity with D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 1-phosphate, D-glucose 6-phosphate, lithium hydroxypyruvate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, D-ribose 5-phosphate, and D-xylose 5-phosphate Methylomonas sp. M15 / DSM 580 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 22000, SDS-PAGE Methylomonas sp.

Synonyms

Synonyms Comment Organism
3-hexulose-phosphate synthase
-
 Methylomonas sp.
HPS
-
 Methylomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Methylomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 remains stable at pH 6.0-8.0 at 30°C for 1 h, enzyme stability decreases at pH values below 6.0 Methylomonas sp.