Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.95 | - |
formaldehyde | recombinant HPS, at 80°C | Pyrococcus horikoshii | |
2.31 | - |
formaldehyde | recombinant HPS, at 60°C | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
recombinant HPS, gel filtration | Pyrococcus horikoshii |
27000 | - |
recombinant HPS, SDS-PAGE | Pyrococcus horikoshii |
47000 | - |
4 * 47000, HPS-PHI fusion enzyme, SDS-PAGE | Pyrococcus horikoshii |
162000 | - |
EDTA-solubilized HPS-PHI fusion enzyme, gel filtration | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus horikoshii | key enzyme of the ribulose monophosphate pathway | ? | - |
? | |
additional information | Pyrococcus horikoshii OT-3 | key enzyme of the ribulose monophosphate pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
strain OT3 | - |
Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and ammonium sulfate precipitation | Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56.7 | - |
crude cell extract | Pyrococcus horikoshii |
235 | - |
after 4.14fold purification | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + formaldehyde | - |
Pyrococcus horikoshii | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
D-ribulose 5-phosphate + formaldehyde | - |
Pyrococcus horikoshii OT-3 | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
additional information | key enzyme of the ribulose monophosphate pathway | Pyrococcus horikoshii | ? | - |
? | |
additional information | key enzyme of the ribulose monophosphate pathway | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 47000, HPS-PHI fusion enzyme, SDS-PAGE | Pyrococcus horikoshii |
monomer | 1 * 27000, recombinant HPS, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
3-hexulose-6-phosphate synthase | - |
Pyrococcus horikoshii |
HPS | - |
Pyrococcus horikoshii |
HPS-PHI | bifunctional enzyme with 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities | Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62.2 | - |
formaldehyde | recombinant HPS, at 80°C | Pyrococcus horikoshii | |
159 | - |
formaldehyde | recombinant HPS, at 60°C | Pyrococcus horikoshii |