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Literature summary for 4.1.2.43 extracted from
- The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway (2005), J. Bacteriol., 187, 3636-3642.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3) cells | Pyrococcus horikoshii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.95 | - |
formaldehyde | recombinant HPS, at 80°C | Pyrococcus horikoshii |  |
| 2.31 | - |
formaldehyde | recombinant HPS, at 60°C | Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
recombinant HPS, gel filtration | Pyrococcus horikoshii |
| 27000 | - |
recombinant HPS, SDS-PAGE | Pyrococcus horikoshii |
| 47000 | - |
4 * 47000, HPS-PHI fusion enzyme, SDS-PAGE | Pyrococcus horikoshii |
| 162000 | - |
EDTA-solubilized HPS-PHI fusion enzyme, gel filtration | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pyrococcus horikoshii | key enzyme of the ribulose monophosphate pathway | ? | - |
? | |
| additional information | Pyrococcus horikoshii OT-3 | key enzyme of the ribulose monophosphate pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
strain OT3 | - |
| Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and ammonium sulfate precipitation | Pyrococcus horikoshii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 56.7 | - |
crude cell extract | Pyrococcus horikoshii |
| 235 | - |
after 4.14fold purification | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + formaldehyde | - |
Pyrococcus horikoshii | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| D-ribulose 5-phosphate + formaldehyde | - |
Pyrococcus horikoshii OT-3 | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| additional information | key enzyme of the ribulose monophosphate pathway | Pyrococcus horikoshii | ? | - |
? | |
| additional information | key enzyme of the ribulose monophosphate pathway | Pyrococcus horikoshii OT-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 47000, HPS-PHI fusion enzyme, SDS-PAGE | Pyrococcus horikoshii |
| monomer | 1 * 27000, recombinant HPS, SDS-PAGE | Pyrococcus horikoshii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hexulose-6-phosphate synthase | - |
Pyrococcus horikoshii |
| HPS | - |
Pyrococcus horikoshii |
| HPS-PHI | bifunctional enzyme with 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities | Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 62.2 | - |
formaldehyde | recombinant HPS, at 80°C | Pyrococcus horikoshii | |
| 159 | - |
formaldehyde | recombinant HPS, at 60°C | Pyrococcus horikoshii | |
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Release 2023.1 (January 2023)
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