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Literature summary for 4.1.2.43 extracted from

  • Ferenci, T.; Stroem, T.; Quayle, J.R.
    Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus (1974), Biochem. J., 144, 477-486.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.43

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 49% inhibition at 1 mM Methylococcus capsulatus
Cu2+ 24% inhibition at 1 mM Methylococcus capsulatus
additional information not inhibited by ATP, ADP, AMP, NADH, D-fructose 6-phosphate, and phosphoenolpyruvate Methylococcus capsulatus
Ni2+ 64% inhibition at 1 mM Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 D-arabino-hex-3-ulose 6-phosphate in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 30°C Methylococcus capsulatus
0.083
-
 D-ribulose 5-phosphate in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C Methylococcus capsulatus
0.49
-
 formaldehyde in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C Methylococcus capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 17% as effective as Mg2+ or Mn2+ in promoting synthase activity Methylococcus capsulatus
Mg2+ 5 mM, essential for activity and stability, 3-hexulose phosphate synthase is rapidly and apparently irreversibly denatured by storage in the absence of one of Mg2+ Methylococcus capsulatus
Mn2+ essential for activity and stability Methylococcus capsulatus
Zn2+ 10% as effective as Mg2+ or Mn2+ in promoting synthase activity Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 6 * 49000, gel filtration, at pH 4.6 Methylococcus capsulatus
310000
-
 gel filtration, under conditions of low pH or low ionic strength Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.71
-
 cell free extract, at 30°C Methylococcus capsulatus
69
-
 after 41fold purification, at 30°C Methylococcus capsulatus

Storage Stability

Storage Stability Organism
-15°C, in the presence of 2.5 mM MgCl2, 6 months, remains stable unless repeatedly frozen and thawed Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + formaldehyde
-
 Methylococcus capsulatus D-arabino-hex-3-ulose 6-phosphate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 49000, gel filtration, at pH 4.6 Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
3-hexulose phosphate synthase
-
 Methylococcus capsulatus
D-arabino-3-hexulose 6-phosphate formaldehyde lyase
-
 Methylococcus capsulatus
HPS
-
 Methylococcus capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 3-hexulose phosphate synthase is rapidly inactivated at elevated temperatures, activity is totally lost within 5 min at 60°C Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Methylococcus capsulatus