Literature summary for 4.1.2.42 extracted from

Hirato, Y.; Tokuhisa, M.; Tanigawa, M.; Ashida, H.; Tanaka, H.; Nishimura, K.
Cloning and characterization of D-threonine aldolase from the green alga Chlamydomonas reinhardtii (2017), Phytochemistry, 135, 18-23 .

Search for more literature for 4.1.2.42

Activating Compound

Activating Compound	Comment	Organism	Structure
8-hydroxyquinoline	1 mM, 5% activation	Chlamydomonas reinhardtii

Application

Application	Comment	Organism
synthesis	the enzyme is a powerful tool for the stereospecific synthesis of various beta-hydroxy amino acids in synthetic organic chemistry	Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in recombinant Escherichia coli BL21 (DE3) cells	Chlamydomonas reinhardtii

Inhibitors

Inhibitors	Comment	Organism	Structure
CaCl2	0.1 mM, 87% inhibition	Chlamydomonas reinhardtii
CoCl2	0.1 mM, 12% inhibition	Chlamydomonas reinhardtii
CuCl2	0.1 mM, complete inhibition	Chlamydomonas reinhardtii
EDTA	1 mM, complete inhibition	Chlamydomonas reinhardtii
hydroxylamine	1 mM, 95% inhibition	Chlamydomonas reinhardtii
iodoacetate	1 mM, 11% inhibition	Chlamydomonas reinhardtii
MgCl2	0.1 mM, 24% inhibition	Chlamydomonas reinhardtii
NiCl2	0.1 mM, 16 % inhibition	Chlamydomonas reinhardtii
p-chloromercuribenzoate	1 mM, 7% inhibition	Chlamydomonas reinhardtii
Sodium cyanide	1 mM, 87% inhibition	Chlamydomonas reinhardtii
ZnCl2	0.1 mM, complete inhibition	Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.31	-	D-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii
0.81	-	D-threo-phenylserine	pH 8.1, 50°C	Chlamydomonas reinhardtii
2.4	-	D-allo-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	A0A1C9ZZ39	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.05	-	pH 8.1, 50°C	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-allo-threonine	-	Chlamydomonas reinhardtii	glycine + acetaldehyde	-	r
D-threo-phenylserine	-	Chlamydomonas reinhardtii	benzaldehyde + glycine	-	r
D-threonine	-	Chlamydomonas reinhardtii	glycine + acetaldehyde	-	r
glycine + acetaldehyde	the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18%	Chlamydomonas reinhardtii	D-allothreonine	-	r
glycine + acetaldehyde	the enzyme catalyzes the synthesis of D- and D-allothreonine from a mixture of glycine and acetaldehyde. The diastereomer excess of D-threonine is 18%	Chlamydomonas reinhardtii	D-threonine	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	cleavage of D-threonine	Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	1 h, 90% of residual activity	Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2	-	D-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii
25	-	D-allo-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii
280	-	D-threo-phenylserine	pH 8.1, 50°C	Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.4	-	cleavage of D-threonine, in 0.1 M HEPES-NaOH buffer	Chlamydomonas reinhardtii

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	pyridoxal 5'-phosphate-dependent enzyme	Chlamydomonas reinhardtii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
11	-	D-allo-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii
13	-	D-threonine	pH 8.1, 50°C	Chlamydomonas reinhardtii
350	-	D-threo-phenylserine	pH 8.1, 50°C	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)