Application | Comment | Organism |
---|---|---|
pharmacology | efficient, environmentally friendly process for the production of (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid by a recombinant D-threonine aldolase catalyzed aldol addition of glycine and pyridine 4-carboxaldehyde. (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid, is a key intermediate in the synthesis of the (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-1-(pyrrolidin-1-yl)propan-1-one, a developmental drug candidate. The aldol addition product directly crystallizes out from the reaction mixture in high purity and high diastereo- and enantioselectivity, contributing to high yield and allowing easy isolation, processing, and downstream utilization | Arthrobacter sp. |
pharmacology | efficient, environmentally friendly process for the production of (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid by a recombinant D-threonine aldolase catalyzed aldol addition of glycine and pyridine 4-carboxaldehyde. (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid, is a key intermediate in the synthesis of the (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-1-(pyrrolidin-1-yl)propan-1-one, a developmental drug candidate. The aldol addition product directly crystallizes out from the reaction mixture in high purity and high diastereo- and enantioselectivity, contributing to high yield and allowing easy isolation, processing, and downstream utilization | Achromobacter xylosoxidans |
synthesis | efficient, environmentally friendly process for the production of (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid by a recombinant D-threonine aldolase catalyzed aldol addition of glycine and pyridine 4-carboxaldehyde. (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid, is a key intermediate in the synthesis of the (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-1-(pyrrolidin-1-yl)propan-1-one, a developmental drug candidate. The aldol addition product directly crystallizes out from the reaction mixture in high purity and high diastereo- and enantioselectivity, contributing to high yield and allowing easy isolation, processing, and downstream utilization | Arthrobacter sp. |
synthesis | efficient, environmentally friendly process for the production of (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid by a recombinant D-threonine aldolase catalyzed aldol addition of glycine and pyridine 4-carboxaldehyde. (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid, is a key intermediate in the synthesis of the (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-1-(pyrrolidin-1-yl)propan-1-one, a developmental drug candidate. The aldol addition product directly crystallizes out from the reaction mixture in high purity and high diastereo- and enantioselectivity, contributing to high yield and allowing easy isolation, processing, and downstream utilization | Achromobacter xylosoxidans |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Arthrobacter sp. |
overexpression in Escherichia coli | Achromobacter xylosoxidans |
General Stability | Organism |
---|---|
divalent transition metal cations (cobalt and nickel) improve the stability significantly. Ni2+ is the best and retains almost all activity on storage at 4 °C for a week. Although MnCl2 was required to catalyze the condensation | Arthrobacter sp. |
divalent transition metal cations (cobalt and nickel) improve the stability significantly. Ni2+ is the best and retains almost all activity on storage at 4 °C for a week. Although MnCl2 was required to catalyze the condensation | Achromobacter xylosoxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | rrequired for condensation reaction | Arthrobacter sp. | |
MnCl2 | rrequired for condensation reaction | Achromobacter xylosoxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Arthrobacter sp. |
42000 | - |
SDS-PAGE | Achromobacter xylosoxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | Q9RBG6 | - |
- |
Achromobacter xylosoxidans IFO 12699 | Q9RBG6 | - |
- |
Arthrobacter sp. | O82872 | - |
- |
Arthrobacter sp. DK-38 | O82872 | - |
- |
Storage Stability | Organism |
---|---|
4°C, enzyme solution prepared by lysing the cells loses about 55% of its initial activity after 1 week of storage | Arthrobacter sp. |
4°C, enzyme solution prepared by lysing the cells loses about 55% of its initial activity after 1 week of storage | Achromobacter xylosoxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + pyridine-4-carboxaldehyde | - |
Arthrobacter sp. | (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid | - |
? | |
glycine + pyridine-4-carboxaldehyde | - |
Achromobacter xylosoxidans | (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid | - |
? | |
glycine + pyridine-4-carboxaldehyde | - |
Arthrobacter sp. DK-38 | (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid | - |
? | |
glycine + pyridine-4-carboxaldehyde | - |
Achromobacter xylosoxidans IFO 12699 | (2R,3S)-2-amino-3-hydroxy-3-(pyridin-4-yl)-propanoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Arthrobacter sp. |
monomer | - |
Achromobacter xylosoxidans |
Synonyms | Comment | Organism |
---|---|---|
ARDTA | - |
Arthrobacter sp. |
AXDTA | - |
Achromobacter xylosoxidans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | rrequired | Arthrobacter sp. | |
pyridoxal 5'-phosphate | rrequired | Achromobacter xylosoxidans |