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Literature summary for 4.1.2.4 extracted from

  • Woo, M.; Kim, M.; Chung, N.; Kim, J.
    Expression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20 (2014), J. Korean Soc. Appl. Biol. Chem., 57, 655-660 .
No PubMed abstract available
Search for more literature for 4.1.2.4

Application

Application Comment Organism
synthesis synthesis of statin intermediates. The recombinant enzyme can be potentially applied in the production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose. The bioconversion process for production of (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant enzyme is studied and this process took 3 h for maximal conversion Haemophilus influenzae

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, temperature not specified in the publication Haemophilus influenzae
24.77
-
 2-deoxy-D-ribose pH 7.5, temperature not specified in the publication Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 SDS-PAGE Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae P44430
-
-
Haemophilus influenzae DSM 11121 P44430
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.1
-
 pH 7.5, temperature not specified in the publication Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose
-
 Haemophilus influenzae D-glyceraldehyde + acetaldehyde
-
 r
2-deoxy-D-ribose
-
 Haemophilus influenzae DSM 11121 D-glyceraldehyde + acetaldehyde
-
 r
2-deoxy-D-ribose 5-phosphate
-
 Haemophilus influenzae D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
2-deoxy-D-ribose 5-phosphate
-
 Haemophilus influenzae DSM 11121 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
D-glyceraldehyde 3-phosphate + acetaldehyde 2-deoxy-D-ribose 5-phosphate-producing activity is highest in the presence of 300 mM acetaldehyde Haemophilus influenzae 2-deoxy-D-ribose 5-phosphate
-
 r
D-glyceraldehyde 3-phosphate + acetaldehyde 2-deoxy-D-ribose 5-phosphate-producing activity is highest in the presence of 300 mM acetaldehyde Haemophilus influenzae DSM 11121 2-deoxy-D-ribose 5-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Haemophilus influenzae

Synonyms

Synonyms Comment Organism
2-Deoxyribose-5-phosphate aldolase
-
 Haemophilus influenzae
DERA
-
 Haemophilus influenzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Haemophilus influenzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 10°C: about 40% of maximal activity, 50°C: about 50% of maximal activity Haemophilus influenzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, no loss of activity Haemophilus influenzae
50
-
 1 h, less than 20% loss of activity Haemophilus influenzae
60
-
 1 h, complete loss of activity Haemophilus influenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Haemophilus influenzae

pH Range

pH Minimum pH Maximum Comment Organism
5 12 pH 5.0: about 70% of maximal activity, pH 12.0: about 70% of maximal activity Haemophilus influenzae