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Literature summary for 4.1.2.4 extracted from
- Cloning and characterization of a thermostable 2-deoxy-D-ribose-5-phosphate aldolase from Aciduliprofundum boonei (2011), Afr. J. Biotechnol., 10, 16260-16266.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aciduliprofundum boonei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 2 | 2-deoxy-D-ribose 5-phosphate | pH 5.5, 25°C | Aciduliprofundum boonei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26600 | - |
2 * 26600, calculated and SDS-PAGE | Aciduliprofundum boonei |
| 53000 | - |
gel filtration | Aciduliprofundum boonei |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetaldehyde | 250 mM, 25°C, up to 4 h,70% residual activity | Aciduliprofundum boonei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aciduliprofundum boonei | B5IEU6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Aciduliprofundum boonei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-ribose 5-phosphate | - |
Aciduliprofundum boonei | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26600, calculated and SDS-PAGE | Aciduliprofundum boonei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Aciduliprofundum boonei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, no significant loss of activity | Aciduliprofundum boonei |
| 80 | - |
10 min, no loss of activity | Aciduliprofundum boonei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aciduliprofundum boonei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | at least 75% of maximum activity | Aciduliprofundum boonei |
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